Exploring R2* and R1 as imaging biomarkers of tumor oxygenation
Exploring R2* and R1 as imaging biomarkers of tumor oxygenation
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DOI：
10.1002/jmri.23987
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发表时间：
2013-08-01
影响因子：
4.4
通讯作者：
Robinson, Simon P.
中科院分区：
文献类型：
--
作者：
Burrell, Jake S.;Walker-Samuel, Simon;Robinson, Simon P.
目的：探讨联合使用高氧诱导的R-2* 和R-1作为肿瘤缺氧的非侵入性成像生物标志物。材料和方法：MRI进行大鼠GH 3泌乳素瘤（n = 6）和人PC 3前列腺裸鼠移植瘤（n = 6）繁殖。在Carbogen治疗前和治疗期间，从相同的横切片采集多梯度回波和反转恢复TrueFISP图像，以定量肿瘤R-2* 和R-1（95%O-2/5%CO2）挑战，并评估R-2* 和R-1的相关性。GH 3泌乳素瘤的平均基线R-2* 和R-1分别为119 +/- 7 s（-1）和0.6 +/- 0.03 s（-1），PC 3异种移植物的平均基线R-2* 和R-1分别为77 +/- 12 s（-1）和0.7 +/- 0.02 s（-1）。在碳氧呼吸期间，GH 3的平均R-2* 和R-1分别为-20 +/- 8 s（-1）和0.08 +/- 0.03 s（-1），PC 3肿瘤的平均R-2* 和R-1分别为-0.5 +/- 1 s（-1）和0.2 +/- 0.08 s（-1）。结论：考虑到血氧-血红蛋白解离曲线，快速R-2* 表明GH 3泌乳素瘤在基线时更缺氧，其碳源反应主要由血红蛋白氧合增加决定，证明了高度负R-2*。PC 3肿瘤在基线时缺氧较少，它们对碳源的反应主要是溶解氧增加，这由高度阳性的R-1证明。由于这两种生物标志物对不同的氧合范围敏感，因此R-2* 和R-1的组合可能比单独使用更好地表征肿瘤缺氧。J.磁共振Imaging 2013;38：429-434. (c)2013 Wiley Periodicals，Inc.
Purpose: To investigate the combined use of hyperoxia-induced R-2* and R-1 as a noninvasive imaging biomarker of tumor hypoxia.Materials and Methods: MRI was performed on rat GH3 prolactinomas (n = 6) and human PC3 prostate xenografts (n = 6) propagated in nude mice. multiple gradient echo and inversion recovery truefisp images were acquired from identical transverse slices to quantify tumor R-2* and R-1 before and during carbogen (95% O-2/5% CO2) challenge, and correlates of R-2* and R-1 assessed.Results: Mean baseline R-2* and R-1 were 119 +/- 7 s(-1) and 0.6 +/- 0.03 s(-1) for GH3 prolactinomas and 77 +/- 12 s(-1) and 0.7 +/- 0.02 s(-1) for PC3 xenografts, respectively. During carbogen breathing, mean R-2* and R-1 were -20 +/- 8 s(-1) and 0.08 +/- 0.03 s(-1) for GH3 and -0.5 +/- 1 s(-1) and 0.2 +/- 0.08 s(-1) for the PC3 tumors, respectively. A pronounced relationship between R-2* and R-1 was revealed.Conclusion: Considering the blood oxygen-hemoglobin dissociation curve, fast R-2* suggested that GH3 prolactinomas were more hypoxic at baseline, and their carbogen response dominated by increased hemoglobin oxygenation, evidenced by highly negative R-2*. PC3 tumors were less hypoxic at baseline, and their response to carbogen dominated by increased dissolved oxygen, evidenced by highly positive R-1. Because the two biomarkers are sensitive to different oxygenation ranges, the combination of R-2* and R-1 may better characterize tumor hypoxia than each alone. J. Magn. Reson. Imaging 2013;38:429-434. (c) 2013 Wiley Periodicals, Inc.