Characterization, efficient transformation and regeneration of Chirita pumila (Gesneriaceae), a potential evo-devo model plant
Characterization, efficient transformation and regeneration of Chirita pumila (Gesneriaceae), a potential evo-devo model plant
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潜在的 evo-devo 模型植物 Chirita pumila（苦苣苔科）的表征、高效转化和再生
DOI：
10.1007/s11240-014-0488-2
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发表时间：
2014-08-01
影响因子：
3
通讯作者：
Wang, Yin-Zheng
中科院分区：
文献类型：
--
作者：
Liu, Bo-Ling;Yang, Xia;Wang, Yin-Zheng
在进化发育生物学（evo-devo）领域中，高效的转化和再生系统对于发育基因及其相关元件的功能研究至关重要。矮唇柱苣苔Don属于苦苣苔科（Gesneriaceae），是唇形科（Lamiales sensu lato）最基本的类群之一，具有一年生、植株小、世代时间短、后代多、染色体数目少等生物学特性。此外，C. pumila具有闭花受精的花，具有潜在的异花授粉，这是本文首次报道的苦苣苔科的特殊现象。影响芽诱导和遗传转化的参数进行了评估，包括植物生长调节剂，温度，抗生素浓度，预培养和共培养时间，农杆菌细胞密度和感染时间。对T0和T1代植株进行PCR和GUS活性检测，结果表明GUS基因已导入宿主，并在宿主中稳定表达。通过对野生大豆GsNST 1B基因的转化，进一步证实了该转化体系在基因功能研究中的适用性。 L.
An efficient transformation and regeneration system is essential for functional investigation of developmental genes and related elements in the field of evolutionary developmental biology (evo-devo). Chirita pumila D. Don belongs to the Gesneriaceae family, one of the most basal groups in Lamiales sensu lato, and possesses many tractable biological features including annual habit, small plant size, short generation time, abundant offspring and low chromosome number. In addition, C. pumila has cleistogamous flowers with potential cross-pollination, a special phenomenon first reported herein in Gesneriaceae. Parameters affecting shoot induction and genetic transformation have been evaluated, including plant growth regulators, temperature, antibiotic concentration, pre- and co-culture duration, Agrobacterium cell density and infection time. Polymerase chain reaction and β-Glucuronidase (GUS) activity assays of T0 and T1 plants show that the GUS gene has been introduced into the host with stable and universal expression. The applicability of the transformation system in gene function investigation is further confirmed by transforming a GsNST1B gene from Glycine soja. l.