Radioimmunoassay of free genistein in human serum.
Radioimmunoassay of free genistein in human serum.
复制标题
人血清中游离金雀异黄酮的放射免疫测定。
DOI：
10.1016/s0960-0760(97)00199-4
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Adlercreutz,H
中科院分区：
文献类型：
--
作者：
Lapcík,O;Hampl,R;Hill,M;Wähälä,K;Maharik,NA;Adlercreutz,H
基于染料木素-4′- o -（羧甲基）醚-牛血清白蛋白和染料木素-7- o -（羧甲基）醚-牛血清白蛋白偶联物的多克隆抗体，建立了染料木素放射免疫测定（RIA）系统。测定方法的灵敏度分别为4.44和10.4fmol(1.2和2.8pg)/管，测定内变异系数为3.54 ~ 9.30%，测定间变异系数为6.72 ~ 19.7%，与方法类型和染料木素浓度有关。通过染料木素的4′-和7-位制备的试剂，与其他化学相关化合物（用于构建免疫原的染料木素衍生物除外）的交叉反应性分别为5.5和6.1%，与刺芒柄花素的交叉反应性分别为3.9和0.04%。该方法被用于测量26名杂食者和3名志愿者在食用125克煮熟的全大豆后的染料木素水平。在两种RIA系统中，从人血清中获得的乙醚提取物的值几乎相同，表明两种RIA测量的是同一实体。
Two radioimmunoassay (RIA) systems for genistein have been established, based on polyclonal antibodies against genistein-4′-O-(carboxymethyl)ether-bovine serum albumin and genistein-7-O-(carboxymethyl)ether-bovine serum albumin conjugates. The sensitivities of assays were 4.44 and 10.4fmol (1.2 and 2.8pg)/tube, respectively, the intraassay coefficients of variation ranged from 3.54 to 9.30%, the interassay C.V. varied from 6.72 to 19.7%, depending on the type of method and on genistein concentration. The cross-reactivities with other chemically related compounds (with exception of genistein derivatives at the position used for construction of the immunogen) were 5.5 and 6.1% for daidzein and 3.9 and 0.04% for formononetin in RIAs using reagents prepared through positions 4′- and 7- of genistein, respectively. The method was used for measurement of genistein levels in 26 omnivore subjects and in three volunteers after consumption of a meal prepared from 125g of cooked whole soybeans. The values obtained in ether extracts from human sera were almost identical for both RIA systems, indicating that both RIAs measure the same entity.