Antimicrobial Susceptibility and Methicillin Resistance in Staphylococcus pseudintermedius and Staphylococcus schleiferi subsp coagulans Isolated from Dogs with Pyoderma in Japan
Antimicrobial Susceptibility and Methicillin Resistance in Staphylococcus pseudintermedius and Staphylococcus schleiferi subsp coagulans Isolated from Dogs with Pyoderma in Japan
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DOI：
10.1292/jvms.10-0172
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发表时间：
2010-12-01
影响因子：
1.2
通讯作者：
Fukata, Tsuneo
中科院分区：
文献类型：
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作者：
Kawakami, Tetsuji;Shibata, Sanae;Fukata, Tsuneo
为了解日本犬葡萄球菌的种属分布、抗菌药物敏感性趋势和甲氧西林耐药率，从日本2家兽医转诊医院的化脓性链球菌病犬中分离出190株凝固酶阳性葡萄球菌（CoPS）。采用多重聚合酶链反应（M-PCR）方法，鉴定了两种CoPS：170株假中间葡萄球菌（89.5%）和20株S。斯氏亚种凝结物分离株占10.5%。在这些菌株中，7种抗菌药物的敏感性进行了测定。头孢氨苄（CEX）、阿莫西林/克拉维酸（CVA/AMPC）、米诺环素（MINO）、氧氟沙星（OFLX）、诺氟沙星（NFLX）、林可霉素（LCM）和克林霉素（CLDM）对S.假中间体分离株分别为38.2、52.4、34.7、31.2、34.1、1.2和11.2%。In S.斯氏亚种对CEX的敏感率为55%，对CVA/AMPC的敏感率为80%。70%为MIND，45%为OFLX或NFLX，30%为CLDM。没有S。斯氏亚种凝块菌对LCM敏感。为了确定耐甲氧西林菌株的流行情况，我们使用PCR方法，该方法能够在66.5%（113/170）的S. pseudintermedius和30.0%（6/20）的S.斯氏亚种凝结物分离物。耐甲氧西林的CoPS对CEX、CVA/AMPC、OFLX、NFLX和CLDM的敏感率明显低于敏感株。这些数据表明，在日本从感染脓毒症的母犬中分离出的葡萄球菌对甲氧西林具有高水平的耐药性。
To understand species distribution, trends of antimicrobial susceptibility and prevalence of methicillin resistance in canine staphylococci in Japan, 190 coagulase-positive staphylococci (CoPS) were isolated from dogs with pyoderma in 2 Japanese veterinary referral hospitals. Using a multiplex polymerase chain reaction (M-PCR) method, two CoPS species were identified: 170 Staphylococcus pseudintermedius (89.5%) and 20 S. schleiferi subsp. coagulans isolates (10.5%). In these isolates, susceptibility to 7 antimicrobial agents was determined. Overall, the levels of susceptibility to cefalexin (CEX), amoxicillin/clavulanic acid (CVA/AMPC), minocycline (MINO), ofloxacin (OFLX), norfloxacin (NFLX), lincomycin (LCM) and clindamycin (CLDM) in S. pseudintermedius isolates were 38.2, 52.4, 34.7, 31.2, 34.1, 1.2 and 11.2%, respectively. In S. schleiferi subsp. coagulans isolates, 55% demonstrated susceptibility to CEX, 80% to CVA/AMPC. 70% to MIND, 45% to OFLX or NFLX and 30% to CLDM. None of S. schleiferi subsp. coagulans isolates was susceptible to LCM. To determine the prevalence of methicillin-resistant strains, we used a PCR method, which enabled detection of the fragment of mecA gene in 66.5% (113 of 170) in S. pseudintermedius and 30.0% (6 of 20) in S. schleiferi subsp. coagulans isolates. The frequencies of susceptibility to CEX, CVA/AMPC, OFLX, NFLX and CLDM were significantly lower in methicillin-resistant CoPS than in methicillin-susceptible CoPS isolates. These data suggest a high level of methicillin resistance in staphylococci isolated from does with pyoderma in Japan.