Ratiometric Biosensor for Aggregation-Induced Emission-Guided Precise Photodynamic Therapy
Ratiometric Biosensor for Aggregation-Induced Emission-Guided Precise Photodynamic Therapy
复制标题
用于聚集诱导发射引导精确光动力治疗的比例生物传感器
DOI：
10.1021/acsnano.5b04243
复制
发表时间：
2015-10-01
期刊：
影响因子：
17.1
通讯作者：
Zhang, Xian-Zheng
中科院分区：
文献类型：
--
作者：
Han, Kai;Wang, Shi-Bo;Zhang, Xian-Zheng
光动力疗法面临着选择合适照射区域和时间的障碍。在本文中，设计并合成了一种基质金属蛋白酶 - 2（MMP - 2）响应型比率生物传感器，用于聚集诱导发光（AIE）引导的精确光动力治疗。研究发现，该生物传感器呈现出MMP - 2响应的AIE行为。最重要的是，它可以通过游离四苯乙烯和原卟啉IX（PpIX，内参比）之间的荧光比率准确区分肿瘤细胞和健康细胞。体内研究表明，该生物传感器能够在肿瘤组织中优先富集，且血液滞留时间相对较长。值得注意的是，PpIX的固有荧光和MMP - 2触发的AIE荧光提供了实时反馈，有效地指导了体内精确光动力治疗。本文所展示的策略为精准医学，尤其是光疗，打开了一扇窗。
Photodynamic therapy faces the barrier of choosing the appropriate irradiation region and time. In this paper, a matrix metalloproteinase-2 (MMP-2) responsive ratiometric biosensor was designed and synthesized for aggregation-induced emission (AIE)-guided precise photodynamic therapy. It was found that the biosensor presented the MMP-2 responsive AIE behavior. Most importantly, it could accurately differentiate the tumor cells from the healthy cells by the fluorescence ratio between freed tetraphenylethylene and protoporphyrin IX (PpIX, internal reference). In vivo study demonstrated that the biosensor could preferentially accumulate in the tumor tissue with a relative long blood retention time. Note that the intrinsic fluorescence of PpIX and MMP-2-triggered AIE fluorescence provided a real-time feedback which guided precise photodynamic therapy in vivo efficiently. This strategy demonstrated here opens a window in the precise medicine, especially for phototherapy.