Placental biomarkers and angiogenic factors in oral fluids of patients with preeclampsia
Placental biomarkers and angiogenic factors in oral fluids of patients with preeclampsia
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DOI：
10.1002/pd.4811
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发表时间：
2016-05-01
影响因子：
3
通讯作者：
Enrique Illanes, Sebastian
中科院分区：
文献类型：
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作者：
Chaparro, Alejandra;Gaedechens, Dominique;Enrique Illanes, Sebastian
目的本研究的目的是探讨测量唾液和龈沟液（GCF）中内皮和胎盘生物标志物的可行性，并确定先兆子痫（PE）患者口腔液中这些生物标志物的特征是否不同。包括PE患者（n = 10）和在智利圣地亚哥的Soconut德尔里奥医院随机选择的正常妊娠的对照组（n = 20）。在同一胎龄进行完整的牙周和产科病史，包括收集口腔液。通过ELISA测定确定Cd 63（+）细胞外囊泡、胎盘碱性磷酸酶（PLAP）、胎盘生长因子（PlGF）和sFlt-1水平。数据分析采用卡方检验或Fisher精确检验，连续变量采用Mann-Whitney U检验。结果PE患者唾液和龈沟液中sFlt-1浓度显著高于对照组（分别为p = 0.045和p = 0.033）。PE患者GCF中PLAP浓度升高（p = 0.049）。PE患者GCF中的PLAP/CD 63（+）比值显著较高（p = 0.0008）。结论PE患者龈沟液中聚集了较高水平的与PE发生发展相关的生物标志物。(C)2016约翰威利父子有限公司
Objectives The objectives of this study are to explore the feasibility of measuring endothelial and placental biomarkers in saliva and gingival crevicular fluid (GCF) and to determine if patients with preeclampsia (PE) have a different profile of these biomarkers in oral fluids.Method A case-control study was conducted, including patients with PE (n = 10) and a control group with normal pregnancies randomly selected (n = 20) admitted at the Sotero del Rio Hospital in Santiago, Chile. A complete periodontal and obstetric history that involved the collection of oral fluids was performed at the same gestational age. Levels of Cd63(+) extracellular vesicles, placental alkaline phosphatase (PLAP), placental growth factor (PlGF), and sFlt-1 levels were determined by ELISA assays. Data analysis was performed with chi-square or Fisher's exact test, and Mann-Whitney U-test for continuous variables. The association was assessed using a multiple logistic regression model.Results sFlt-1 concentrations in saliva and GCF were significantly higher in patients with PE (p = 0.045 and p = 0.033 respectively). Concentrations of PLAP were elevated in GCF of patients with PE (p = 0.049). The PLAP/CD63(+) ratio in GCF of patients with PE was significantly higher (p = 0.0008). No differences in PlGF levels were observed.Conclusion(s) GCF of patients with PE concentrates higher levels of biomarkers related with the PE development. (C) 2016 John Wiley & Sons, Ltd.