Phylogenetic relatedness and genetic diversity of hepatitis B virus isolates in eastern India
Phylogenetic relatedness and genetic diversity of hepatitis B virus isolates in eastern India
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DOI：
10.1002/jmv.20677
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发表时间：
2006-09-01
影响因子：
12.7
通讯作者：
Chakravarty, Runu
中科院分区：
文献类型：
--
作者：
Banerjee, Arup;Kurbanov, Fuat;Chakravarty, Runu
B型肝炎病毒（HBV）可分为8种基因型和几种亚型，其地理分布具有明显差异。基因型A和D以前被报道在印度占主导地位。最近的研究表明，在印度东部的C基因型的流通的证据。为了确定加尔各答（印度东部人口最多的城市）流行的HBV的系统发育关系和分子遗传学特征，分离了11株HBV，并对全基因组序列进行了分析。确定系统发育分析; 3株与东南亚C1（Cs）亚型参考品密切相关的C型（adr-血清型）分离株和3株与A1（Aa）亚型亚洲变异参考品相关的A型（adw 2-血清型）分离株。然而，5个基因型D（ayw 2，ayw 3血清型）分离株是高度分歧的，一个是相关的亚基因型D1，两个亚基因型D3，和其余两个聚类与一个单一的基因型D分离株来自日本属于一个未分类的亚基因型。总之，这两个分离株与HBV D1-D4亚型的核苷酸差异范围为5.0 - 5.49%，可能表明一个新的亚型，我们将其命名为D5。所有的血清型ayw 3的基因型D分离株的表面基因的抗原决定簇的密码子118处的苏氨酸和密码子125处的蛋氨酸的特异性氨基酸取代，以前没有报道在来自印度其他地区的分离株。最后，利用全基因组分析，本研究证实了基因型C在印度东部地区的传播，并证明了印度基因型D的相当大的基因型异质性。
Hepatitis B virus (HBV) has been classified into eight genotypes, and several subgenotypes, distinctly distributed geographically. The genotypes A and D were previously reported to be predominant in India. Recent studies indicated evidence of circulation of genotype C in Eastern part of India. With the aim to confirm the phylogenetic relation and molecular genetic characteristics of the HBV circulating in Kolkata, the most populous city in Eastern India, 11 strains were isolated and the complete genome sequences were analyzed. Phylogenetic analysis determined; three genotype C (adr-serotype) isolates closely related with C1 (Cs) subgenotype references from South East Asia, and three genotype A (adw2-serotype) isolates, related to Asia-variant references of subgenotype A1 (Aa). Whereas, five genotype D (ayw2, ayw3 serotype) isolates were highly divergent; one was related to subgenotype D1, two to subgenotype D3, and the remaining two clustered with a single genotype D isolate from Japan belonging to an unclassified subgenotype. Together, these two isolates differed from HBV D1-D4 subgenotypes by nucleotide differences ranging from 5.0 to 5.49%, probably indicating a new subgenotype, which we designate as D5. All serotype ayw3 of genotype D isolates had specific amino acid substitution Threonine at codon 118 and Methionine at codon 125 in antigenic determinant of surface gene that has not been reported previously in isolates from other parts of India. In conclusion; using the complete genome analyses this study has confirmed circulation of the genotype C in Eastern part of India and demonstrated considerable genotypic heterogeneity of the Indian genotype D.