L-ARABINOSE PERMEASE SYSTEM IN ESCHERICHIA COLI B/R
L-ARABINOSE PERMEASE SYSTEM IN ESCHERICHIA COLI B/R
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DOI：
10.1016/0304-4165(66)90169-3
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发表时间：
1966-01-01
期刊：
影响因子：
--
通讯作者：
ENGLESBERG, E
中科院分区：
文献类型：
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作者：
NOVOTNY, CP;ENGLESBERG, E
通过测量无 L-阿拉伯糖异构酶突变体对 L-[I-C14]阿拉伯糖的摄取来表征大肠杆菌 B/r 的 L-阿拉伯糖通透酶系统。该生物体通过可诱导的、能量依赖的系统积累L-阿拉伯糖，该系统表现出渗透酶介导的运输的动力学特性。该系统至少由两部分组成：遵守 Michealis-Menten 动力学的入口反应和具有高温度系数且表现出一级动力学的出口反应。抑制研究表明，该系统还可能介导 D-木糖、D-岩藻糖和 D-半乳糖的转运。描述了导致积累的碳水化合物快速损失的“冷休克”现象。
The L-arabinose permease system of Escherichia coli B/r was characterized by measuring L-[I-C14]arabinose uptake by an L-arabinose isomerase-less mutant. This organism accumulates L-arabinose via an inducible, energy-dependent system that exhibits the kinetic properties of permease-mediated transport. The system consists of at least 2 parts: an entrance reaction that obeys Michealis-Menten kinetics and an exit reaction with a high temperature coefficient that exhibits first-order kinetics. Inhibition studies indicate that the system may also mediate the transport of D-xylose, D-fucose, and D-galactose. A "cold shock" phenomenon which results in a rapid loss in accumulated carbohydrates is described.