Hydrophilic surface modification of PDMS for droplet microfluidics using a simple, quick, and robust method via PVA deposition
Hydrophilic surface modification of PDMS for droplet microfluidics using a simple, quick, and robust method via PVA deposition
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DOI：
10.1038/micronano.2016.91
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发表时间：
2017-04-24
影响因子：
7.9
通讯作者：
Ces, Oscar
中科院分区：
文献类型：
--
作者：
Trantidou, Tatiana;Elani, Yuval;Ces, Oscar
聚二甲基硅氧烷（PDMS）由于其高度疏水性，是制备油包水液滴，特别是脂质稳定液滴的微流体装置的主要材料。然而，其疏水性这一关键特性阻碍了其在微流体生成油包水滴中的应用，这就要求通道具有亲水性表面特性。在本文中，我们开发、优化并表征了一种通过等离子体处理后聚乙烯醇沉积来生产具有亲水性表面的PDMS的方法，并证明了其对液滴生成的适用性。所提出的方法简单、快速、有效、低成本，并且在表面活性剂方面用途广泛，使用阴离子表面活性剂和更具生物学相关性的磷脂都能成功生成液滴。该方法还允许该装置选择性地具有亲水性和疏水性区域，从而产生双乳液和反向双乳液。
Polydimethylsiloxane (PDMS) is a dominant material in the fabrication of microfluidic devices to generate water-in-oil droplets, particularly lipid-stabilized droplets, because of its highly hydrophobic nature. However, its key property of hydrophobicity has hindered its use in the microfluidic generation of oil-in-water droplets, which requires channels to have hydrophilic surface properties. In this article, we developed, optimized, and characterized a method to produce PDMS with a hydrophilic surface via the deposition of polyvinyl alcohol following plasma treatment and demonstrated its suitability for droplet generation. The proposed method is simple, quick, effective, and low cost and is versatile with respect to surfactants, with droplets being successfully generated using both anionic surfactants and more biologically relevant phospholipids. This method also allows the device to be selectively patterned with both hydrophilic and hydrophobic regions, leading to the generation of double emulsions and inverted double emulsions.