ALKALINE-PHOSPHATASE ACTIVITY IN THE PERIODONTAL-LIGAMENT AND GINGIVA OF THE RAT MOLAR - ITS RELATION TO CEMENTUM FORMATION
ALKALINE-PHOSPHATASE ACTIVITY IN THE PERIODONTAL-LIGAMENT AND GINGIVA OF THE RAT MOLAR - ITS RELATION TO CEMENTUM FORMATION
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DOI：
10.1177/00220345950740070901
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发表时间：
1995-07-01
影响因子：
7.6
通讯作者：
BEERTSEN, W
中科院分区：
文献类型：
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作者：
GROENEVELD, MC;EVERTS, V;BEERTSEN, W
碱性磷酸酶（ALP）是一种糖蛋白，被认为参与导致骨和牙骨质等组织中矿物质形成的过程。在大鼠磨牙牙周组织中，几个区域与牙骨质（牙周韧带，牙龈的内部）的形成相关，而其他区域则不相关（例如，牙龈的外部正好在外部口腔上皮下面）。在试图建立ALP活性的空间分布与牙骨质形成的关系，我们评估了定量的酶的活性在大鼠上颌磨牙的牙周组织中，通过使用吲哚-四唑盐法。看来，韧带中酶活性的分布是不均匀的，表明磷酸盐家庭的局部变化。最高的活性被发现在矿化相关的区域，邻近牙槽骨和牙骨质。邻近细胞牙骨质的酶活性高于非细胞牙骨质。对于无细胞牙骨质，ALP活性与牙骨质厚度之间存在高度显著的正相关，这表明局部磷酸盐产生与牙骨质形成速率之间存在密切关系。在第一磨牙近中牙龈结缔组织中观察到一个有趣的现象，即在邻近牙齿的ALP阳性内部和在外部口腔上皮下面的ALP阴性外部之间存在明显分界。在齿间牙龈，整个结缔组织被证明是阳性的酶，这表明该地区包括两个相邻牙齿的结合内牙龈部分。
Alkaline phosphatase (ALP) is a glycoprotein thought to be involved in processes leading to mineral formation in tissues like bone and cementum. In the rat molar periodontium, several regions are associated with the formation of cementum (periodontal ligament, inner part of the gingiva), whereas other areas are not (e.g., the outer part of the gingiva just beneath the outer oral epithelium). In an attempt to establish how the spatial distribution of ALP activity relates to cementum formation, we assessed the activity of the enzyme quantitatively in the periodontium of the rat maxillary molars, by using the indoxyl-tetrazolium salt method. It appeared that the distribution of enzyme activity in the Ligament was heterogeneous, indicating local variations in the phosphate household. Highest activity was found in areas related to mineralization, adjacent to the alveolar bone and cementum. Enzyme activity was higher adjacent to cellular cementum than to acellular cementum. With respect to acellular cementum, a highly significant positive correlation was found between ALP activity and cementum thickness, which indicates a close relationship between local phosphate production and cementum formation rate. An interesting observation in the connective tissue of the gingiva mesial to the first molar was a sharp demarcation between an ALP-positive inner part, adjacent to the tooth, and an ALP-negative outer part, underneath the outer oral epithelium. In the interdental gingiva, the entire connective tissue proved positive for the enzyme, suggesting that this region consists of the combined inner gingival parts of two adjacent teeth.