Plant-derived vaccine protects target animals against a viral disease
Plant-derived vaccine protects target animals against a viral disease
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DOI：
10.1038/nbt0397-248
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发表时间：
1997-03-01
影响因子：
46.9
通讯作者：
Rodgers, PB
中科院分区：
文献类型：
--
作者：
Dalsgaard, K;Uttenthal, A;Rodgers, PB
最近已经证实动物或人类病毒表位在植物病毒表面的成功表达。这些嵌合病毒颗粒（cpv）可能是传统动物细胞疫苗的一种具有成本效益和安全性的替代品。我们报道了将水貂肠炎病毒（MEV） VP2衣壳蛋白的短线性表位编码寡核苷酸插入豇豆花叶病毒的感染性cDNA克隆中，并在黑眼豆（Vigna unguiculata）中繁殖时成功地在CVPs表面表达了该表位。在水貂体内皮下注射1毫克的cpv，可以预防临床疾病，并且在受到毒力强的MEV攻击后，几乎可以消除病毒的脱落，这证明了植物cpv作为疫苗开发基础的潜在效用。所使用的抗原表位存在于三种不同的病毒物种中——mev、犬细小病毒和猫泛白细胞减少病毒——因此相同的疫苗可以分别用于三种经济上重要的病毒宿主——水貂、狗和猫。
The successful expression of animal or human virus epitopes on the surface of plant viruses has recently been demonstrated. These chimeric virus particles (CVPs) could represent a cost-effective and safe alternative to conventional animal cell-based vaccines. We report the insertion of oligonucleotides coding for a short linear epitope from the VP2 capsid protein of mink enteritis virus (MEV) into an infectious cDNA clone of cowpea mosaic virus and the successful expression of the epitope on the surface of CVPs when propagated in the black-eyed bean, Vigna unguiculata. The efficacy of the CVPs was established by the demonstration that one subcutaneous injection of 1 mg of the CVPs in mink conferred protection against clinical disease and virtually abolished shedding of virus after challenge with virulent MEV, demonstrating the potential utility of plant CVPs as the basis for vaccine development. The epitope used occurs in three different virus species-MEV, canine parvovirus, and feline panleukopenia virus-and thus the same vaccine could be used in three economically important viral hosts-mink, dogs, and cats, respectively.