Progressive Myofibril Reorganization of Human Cardiomyocytes on a Dynamic Nanotopographic Substrate
Progressive Myofibril Reorganization of Human Cardiomyocytes on a Dynamic Nanotopographic Substrate
复制标题
动态纳米拓扑基质上人心肌细胞的渐进性肌原纤维重组
DOI：
10.1021/acsami.0c03464
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发表时间：
2020
影响因子：
9.5
通讯作者：
Ma, Zhen
中科院分区：
文献类型：
--
作者：
Sun, Shiyang;Shi, Huaiyu;Moore, Sarah;Wang, Chenyan;Ash-Shakoor, Ariel;Mather, Patrick T.;Henderson, James H.;Ma, Zhen
心肌细胞(CM)与横纹肌原纤维组织的排列是在心脏器官发生的早期发展起来的。以前的工作已经成功地实现了使用各种具有静态地形特征的生物材料支架和衬底的体外CM对齐。然而，在CMS对动态表面形态变化的响应过程中发生的细胞过程仍然知之甚少，这可能为胚胎心肌内CM排列的活体发育过程提供一个模型。为了深入了解CMS对动态地形变化的响应中涉及的这些细胞过程，我们开发了一种动态地形衬底，该衬底使用涂有聚电解质多层膜的形状记忆聚合物，当孵化温度变化时，可以产生从平到皱的表面转变。使用该系统，我们研究了细胞形态排列和细胞内肌原纤维重组对动态皱纹形成的响应。因此，我们以时间依赖的方式确定了人类诱导的多能干细胞-CMS的进展细胞过程，这可能为响应细胞外微环境变化的心肌原纤维重组的机制模型提供基础。
Cardiomyocyte (CM) alignment with striated myofibril organization is developed during early cardiac organogenesis. Previous work has successfully achievedin vitroCM alignment using a variety of biomaterial scaffolds and substrates with static topographic features. However, the cellular processes that occur during the response of CMs to dynamic surface topographic changes, which may provide a model ofin vivodevelopmental progress of CM alignment within embryonic myocardium, remains poorly understood. To gain insights into these cellular processes involved in the response of CMs to dynamic topographic changes, we developed a dynamic topographic substrate that employs a shape memory polymer coated with polyelectrolyte multilayers to produce a flat-to-wrinkle surface transition when triggered by a change in incubation temperature. Using this system, we investigated cellular morphological alignment and intracellular myofibril reorganization in response to the dynamic wrinkle formation. Hence, we identified the progressive cellular processes of human-induced pluripotent stem cell-CMs in a time-dependent manner, which could provide a foundation for a mechanistic model of cardiac myofibril reorganization in response to extracellular microenvironment changes.