Human thymus contains IFN-α-producing CD11c-, myeloid CD11c+ and mature interdigitating dendritic cells
Human thymus contains IFN-α-producing CD11c-, myeloid CD11c+ and mature interdigitating dendritic cells
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DOI：
10.1172/jci11734
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发表时间：
2001-04-01
影响因子：
15.9
通讯作者：
Brière, F
中科院分区：
文献类型：
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作者：
Bendriss-Vermare, N;Barthélémy, C;Brière, F
从人胸腺中分离出三种不同的树突状细胞（DC）亚群，它们能够刺激同种异体幼稚T细胞。最丰富的亚群是浆细胞样DC（pDC），其在用灭活流感病毒刺激时分泌大量IFN-α，因此可能对应于最近鉴定的外周血天然IFN-α/β产生细胞（IPC）。与后者细胞一样，胸腺pDC具有独特的表型特征（即，Lin（-）、HLA-DRint、IL-3R α（hi）、CD 45 RA（hi）、CD 11 c（-）、CD 13-和CD 3310），并在IL-3和CD 40 L中培养后发育成成熟DC。在另外两个DC亚群中，一个显示未成熟髓样DC（imDC）（HLA-DRint、CD 111 c（+）、CD 13（+）、CD 33（+））的表型，另一个代表HLA-DRhi CD 11 c（+）成熟DC（mDC）。由于它们也表达DC-LAMP，这些mDC似乎对应于交错树突状细胞（IDC）。胸腺pDC，而不是髓样imDC，强烈表达淋巴特异性转录物，如前-T α，λ-样，和Spi-B，从而表明可能的淋巴起源。不仅在pDC体外成熟时，而且在新鲜纯化的IDC中检测到Spi-B mRNA，表明体内pDC可以分化为IDC。
Three distinct dendritic cell (DC) subsets capable of stimulating allogeneic naive T cells were isolated from human thymus. The most abundant subset was represented by plasmacytoid DCs (pDCs), which secreted high amounts of IFN-alpha upon stimulation with inactivated influenza virus and thus likely correspond to the recentlyidentified peripheral blood natural IFN-alpha/beta -producing cells (IPCs). Like those latter cells, thymic pDCs had distinctive phenotypic features (i.e., Lin(-), HLA-DRint, IL-3R alpha (hi), CD45RA(hi), CD11c(-), CD13-, and CD3310) and developed into mature DCs upon culture in IL-3 and CD40L. Of the two other DC subsets, one displayed a phenotype of immature myeloid DCs (imDCs) (HLA-DRint, CD111c(+), CD13(+), CD33(+)), and the other represented HLA-DRhi CD11c(+) mature DCs (mDCs). Since they also expressed DC-LAMP, these mDCs appear to correspond to interdigitating dendritic cells (IDCs). Thymic pDCs, but not myeloid imDCs, strongly expressed lymphoid-specific transcripts such as pre-T alpha, lambda -like, and Spi-B, thereby suggesting a possible lymphoid origin. The detection of Spi-B mRNA, not only upon in vitro maturation ofpDCs, but also in freshly purified IDCs, suggests that in vivo pDCs may differentiate into IDCs.