Postmortem diagnosis of Fusarium panophthalmitis by the polymerase chain reaction.
Postmortem diagnosis of Fusarium panophthalmitis by the polymerase chain reaction.
复制标题
通过聚合酶链反应进行死后镰刀菌全眼炎诊断。
DOI：
10.1016/s0002-9394(14)70594-x
复制
发表时间：
1996
影响因子：
4.2
通讯作者：
Gloor,P
中科院分区：
文献类型：
--
作者：
Alexandrakis,G;Sears,M;Gloor,P
PurposeWe undertaked this study to determine if a polymerase chain reaction based test that we developed for the conventional fungus，Fusarium，could be used to detect the organisms in postmortem ocular tissues.MethodsWe applied the polymerase chain reaction to amplify a target fragment of FusariumDNA from formalin fixed ocular tissues from a patient with endogenous Fusariumpanophthalmitis.ResultsBy using the polymerase chain reaction based test，我们能够扩增目标片段的DNA从受感染的眼睛，但不是从未感染的控制eyes.ConclusionsThe技术似乎持有承诺是一个敏感，特异性，和快速的方法诊断Fusariuminfections。
PurposeWe undertook this study to determine if a polymerase chain reaction-based test that we developed for the filamentary fungus,Fusarium, could be used to detect the organism in postmortem ocular tissues.MethodsWe applied the polymerase chain reaction to amplify a target fragment ofFusariumDNA from formalin-fixed ocular tissues from a patient with endogenousFusariumpanophthalmitis.ResultsBy using the polymerase chain reaction-based test, we were able to amplify the target fragment of DNA from the infected eyes, but not from uninfected control eyes.ConclusionsThe technique appears to hold promise to be a sensitive, specific, and rapid method of diagnosingFusariuminfections.