Effects of titanium dioxide nanoparticles on red swamp crayfish, Procambarus clarkii: Bioaccumulation, oxidative stress and histopathological biomarkers
Effects of titanium dioxide nanoparticles on red swamp crayfish, Procambarus clarkii: Bioaccumulation, oxidative stress and histopathological biomarkers
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DOI：
10.1016/j.ejar.2019.01.001
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发表时间：
2019-03
期刊：
影响因子：
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通讯作者：
M. A. El-Atti;M. Desouky;A. Mohamadien;R. .. Said
中科院分区：
文献类型：
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作者：
M. A. El-Atti;M. Desouky;A. Mohamadien;R. .. Said
二氧化钛纳米颗粒（TiO2NPs）在许多应用中的广泛使用导致其大量释放到水生系统中。因此，本研究旨在评价tio2纳米粒子对克氏原螯虾（procambarus clarkii）的影响，而克氏原螯虾通常被用作水污染的生物指标。成年雄性标本分别用25、125和250 mg/l的TiO2NPs处理28 天。25、125和250 mg/l TiO2NPs处理的动物死亡率分别为0、3.3和10%。钛在不同组织中的生物蓄积水平如下，鳃 > 肝胰腺 > 绿腺 > 肌肉。TiO2 nps暴露的小龙虾肝胰脏过氧化氢酶、谷胱甘肽- s转移酶、谷胱甘肽过氧化物酶、γ -谷氨酰转移酶、谷胱甘肽和金属硫蛋白水平显著升高。小龙虾暴露于250 mg/l的TiO2NPs中会引起严重的组织病理学改变。在肝胰腺中，最突出的病理改变是小管破坏和炎症浸润。透射电镜下观察到微绒毛破裂、线粒体变形、粗内质网断裂。光镜下观察到鳃片肿胀，上皮细胞紊乱、变性。电镜下观察，250 mg/l tio2纳米粒子处理后，小龙虾鳃呈空泡化，颗粒致密，顶端质膜包涵数减少。综上所述，TiO2NPs引起了抗氧化活性的改变和严重的组织病理学改变。
The widespread use of titanium dioxide nanoparticles (TiO2NPs) in many applications has led to its significant release to aquatic systems. Hence, the present study was performed to evaluate effects of TiO2NPs on crayfishProcambarus clarkii, which is often used as a bioindicator of water pollution. Adult male specimens were treated with 25, 125, and 250 mg/l of TiO2NPs for 28 days. Mortalities were 0, 3.3, and 10% for animals treated with 25, 125, and 250 mg/l of TiO2NPs, respectively. The level of titanium bioaccumulation in different tissues was as follows, gills > hepatopancreas > green glands > muscles. TiO2 NPs-exposed crayfish showed significant increase in the levels of catalase, glutathione-S-transferase, glutathione peroxidase, gamma-glutamyl transferase, glutathione, and metallothioneins in the hepatopancreas. Exposure of the crayfish to 250 mg/l TiO2NPs caused severe histopathological alterations. In the hepatopancreas, the most prominent pathological changes were tubular disruption and inflammatory infiltration. Under transmission electron microscope (TEM), ruptured microvilli, deformed mitochondria and fragmentation of rough endoplasmic reticulum (RER) were observed. In the gills, swelling of gill lamellae, disorganization and degeneration of epithelial cells were noted under the light microscope (LM). Under electron microscope (EM), gills of crayfish treated with 250 mg/l TiO2NPs showed vacuolation, dense granules, and diminution in the number of apical plasma membrane infoldings. In conclusion, TiO2NPs caused alteration in antioxidant activities and severe histopathological changes.