Detection in chimpanzees of a novel flavivirus related to GB virus-C hepatitis G virus
Detection in chimpanzees of a novel flavivirus related to GB virus-C hepatitis G virus
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DOI：
10.1099/0022-1317-79-8-1871
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发表时间：
1998-08-01
影响因子：
3.8
通讯作者：
Simmonds, P
中科院分区：
文献类型：
--
作者：
Adams, NJ;Prescott, LE;Simmonds, P
庚型肝炎病毒（HGV）或GB病毒-C（GBV-C）感染广泛分布于人群中。为了进一步了解GBV-C/HGV与宿主的关系，我们采用套式聚合酶链反应（nested polymerase chain reactions，PCR）技术，从非圈养黑猩猩的5'非编码区（5' non-coding，5 'NC）、非结构区3（non-structural 3，NS 3）和NS 5区（non-structural 3，NS 5）中筛选出GBV-C/HGV相关病毒（Pan troglodytes、troglodytes和verus亚种），从取自39只黑猩猩中的3只的样品和取自一只黑猩猩的NS 3区的样品扩增来自5 'NCR和NS 5区的序列。每个区域的序列比较显示，感染黑猩猩的GB病毒与GBV-C/HGV和任何已知的GBV-A序列都不同，但与人类病毒的关系更密切。从不同黑猩猩中回收的GB病毒比在人类中发现的GBV-C/HGV变体更多样化，在从穴居人和verus亚种中回收的变体之间，5 'NCR中的序列差异为25%，NS 5中的序列差异为20%（9.5%氨基酸），而感染人类的GBV-C/HGV变体之间的差异为7.4%和10.4%（1.9%氨基酸）。在旧世界猴种中发现GBV-C/HGV相关病毒表明OS样病毒可能广泛分布于猿猴中，并表明与其天然宿主有密切的进化关系。
Infection with hepatitis G virus (HGV) or GB virus-C (GBV-C) is widely distributed in human populations. Viruses related to GBV-C/HGV have been recovered from several New World primate species, including tamarins, owl monkeys and marmosets, To understand more about the relationship between GB viruses and their hosts, we used primers from the 5' non-coding (5'NC), non-structural 3 (NS3) and NS5 regions in nested polymerase chain reactions to screen for related viruses infecting non-captive chimpanzees (Pan troglodytes, troglodytes and verus subspecies), Sequences from the 5'NCR and NS5 regions were amplified from samples taken from 3 of 39 chimpanzees, and from one chimpanzee in the NS3 region. Sequence comparisons of each region revealed that the GB virus infecting chimpanzees was distinct from both GBV-C/HGV and from any of the known GBV-A sequences, but was more closely related to human viruses. GB viruses recovered from different chimpanzees were more diverse than variants of GBV-C/HGV found in humans, with 25% sequence divergence in the 5'NCR and 20% (9.5% amino acid) sequence divergence in NS5 between variants recovered from the troglodytes and verus subspecies, compared with 7.4% and 10.4% (1.9% amino acid) divergence amongst GBV-C/HGV variants infecting humans. Finding GBV-C/HGV-related viruses in an Old World monkey species suggests that OS-like viruses may be widely distributed in simians, and suggests a close evolutionary relationship with their natural hosts.