QTL analysis for rice stripe resistance in the Japanese Upland Rice Kanto72
QTL analysis for rice stripe resistance in the Japanese Upland Rice Kanto72
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DOI：
10.1270/jsbbs.54.19
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发表时间：
2004-03-01
期刊：
影响因子：
2.4
通讯作者：
Sunohara, Y
中科院分区：
文献类型：
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作者：
Maeda, H;Sugisawa, T;Sunohara, Y
许多日本旱稻品种表现出对水稻条纹病毒(RSV)的高度抗性。这种抗性被认为是由一对互补的显性基因Stva和Stvb控制的。以感病品种日本晴与抗病品系旱稻关东72(URK72)杂交的120个F-2株/F-3品系为材料，对水稻条纹叶枯病抗性进行了QTL分析，在第2、11染色体上检测到两个QTL，并将这两个QTL的染色体区域导入两个抗病水稻品系Chugoku40和Chugoku41中。为了验证这两个QTL，我们从F2植株中选择了两个信息量较大的植株NR8(11号染色体QTL上的杂合)和NR23(2号染色体上的QTL上的杂合)，并利用NR23(99株)和NR8(96株)的自交后代进行了进一步的QTL分析。在这些二次分析中，一个QTL被定位在第11染色体上的RFLP标记G257附近，另一个QTL被定位在第2染色体上的SSR标记MS-11附近。由于Stvb基因与定位在第11染色体上的另一个抗RSV基因Stvb-I等位，因此认为在第11染色体上检测到的QTL与Stvb基因相对应。然而，该QTL似乎比以前报道的对RSV抗性有更大的影响，并被发现有助于抑制RSV感染。另一方面，由于2号染色体上检测到的QTL不能抑制RSV的侵染，因此它可能增强了11号染色体上的QTL，抑制了RSV感染后的症状。
Many Japanese upland rice varieties show a high level of resistance to rice stripe virus (RSV). This resistance is thought to be controlled by a pair of complementary dominant genes, Stva and Stvb. Quantitative trait locus (QTL) analysis for the rice stripe virus resistance was performed using 120 F-2 plants/ F-3 lines derived from a cross between the susceptible variety Nipponbare, and the resistant line Upland Rice Kanto72 (URK72), As a result, two QTLs were detected on chromosomes 2 and 11. The chromosomal regions of the two QTLs were introgressed into two resistant paddy lines Chugoku40 and Chugoku41, which were developed by introducing resistance genes from URK72. In order to verify the two QTLs, we selected two informative plants NR8 (heterozygous at the QTL on chromosome 11) and NR23 (heterozygous at the QTL on chromosome 2), from F2 plants and we used the self-pollinated progenies of NR23 (99 plants) and NR8 (96 plants) for further QTL analyses. In these secondary analyses, one QTL was mapped near the RFLP marker G257 on chromosome 11 and the other QTL was mapped near SSR marker MS-11 on chromosome 2. Since the Stvb gene is allelic with the other RSV resistance gene Stvb-i, mapped on chromosome 11, the QTL detected on chromosome 11 was thought to correspond to the Stvb gene. However, this QTL seemed to have a larger effect on RSV resistance than previously reported and was found to contribute to the suppression of RSV infection. On the other hand, since the QTL detected on chromosome 2 did not suppress the RSV infection, it probably enhanced the QTL on chromosome 11 and suppressed the symptoms after infection by RSV.