Microbial abundance, composition, and function in nectar are shaped by flower visitor identity.
Microbial abundance, composition, and function in nectar are shaped by flower visitor identity.
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DOI：
10.1093/femsec/fiaa003
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发表时间：
2020-01
影响因子：
4.2
通讯作者：
Megan M. Morris;Natalie J Frixione;Alexander Burkert;E. Dinsdale;R. Vannette
中科院分区：
文献类型：
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作者：
Megan M. Morris;Natalie J Frixione;Alexander Burkert;E. Dinsdale;R. Vannette
微生物的扩散是建立新的栖息地必不可少的，但在社区组装和功能的载体身份的作用知之甚少。在这里，我们比较了微生物的组装和功能的花花蜜访问合法的传粉者（蜂鸟）和花蜜强盗（木匠蜂）。我们使用鸟枪宏基因组学和花蜜化学评估了访问对可培养细菌和真菌的丰度和组成及其分类和功能的影响。我们还比较了宏基因组组装的基因组（MAG）的不动杆菌，一种常见的和高度丰富的花蜜细菌，游客治疗。访问增加了微生物丰度，但抢劫导致10倍以上的微生物丰度比授粉。微生物群落不同游客治疗：抢劫花的特点是主要的花蜜专家内醋柳科和Metschnikowiaceae，与罕见的类群的同时损失，这些产生的社区窝藏有关的渗透胁迫，糖代谢，和专门的转运基因。基因差异反映在功能上：被掠夺的花蜜含有25%以上的单糖。草案基因组不动杆菌揭示了不同的氨基酸和糖类利用途径的菌株从抢劫与授粉花。我们的研究结果表明，一个未被承认的成本花蜜抢劫授粉和不同的游客类型的植物和传粉者之间的相互作用的影响。总的来说，这些结果表明，载体身份是一个未得到充分重视的因素，构建微生物群落组装和功能。
Microbial dispersal is essential for establishment in new habitats, but the role of vector identity is poorly understood in community assembly and function. Here, we compared microbial assembly and function in floral nectar visited by legitimate pollinators (hummingbirds) and nectar robbers (carpenter bees). We assessed effects of visitation on the abundance and composition of culturable bacteria and fungi and their taxonomy and function using shotgun metagenomics, and nectar chemistry. We also compared metagenome-assembled genomes (MAGs) of Acinetobacter, a common and highly abundant nectar bacteria, among visitor treatments. Visitation increased microbial abundance, but robbing resulting in 10x higher microbial abundance than pollination. Microbial communities differed among visitor treatments: robbed flowers were characterized by predominant nectar specialists within Acetobacteraceae and Metschnikowiaceae, with a concurrent loss of rare taxa, and these resulting communities harbored genes relating to osmotic stress, saccharide metabolism, and specialized transporters. Gene differences were mirrored in function: robbed nectar contained 25% more monosaccharides. Draft genomes of Acinetobacter revealed distinct amino acid and saccharide utilization pathways in strains isolated from robbed versus pollinated flowers. Our results suggest an unrecognized cost of nectar robbing for pollination and distinct effects of visitor type on interactions between plants and pollinators. Overall, these results suggest vector identity is an underappreciated factor structuring microbial community assembly and function.