Controlled synchronization of prolactin/STAT5 and AKT1/mTOR in bovine mammary epithelial cells
Controlled synchronization of prolactin/STAT5 and AKT1/mTOR in bovine mammary epithelial cells
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牛乳腺上皮细胞中催乳素/STAT5 和 AKT1/mTOR 的受控同步
DOI：
10.1007/s11626-020-00432-x
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发表时间：
2020-02
影响因子：
2.1
通讯作者：
Zhao Feng
中科院分区：
文献类型：
--
作者：
Wang Baosheng;Shi Linlin;Men Jingjing;Li Qingzhang;Hou Xiaoming;Wang Chunmei;Zhao Feng
催乳素/STAT 5和AKT 1/mTOR通路分别在乳蛋白的转录和翻译中起关键作用。然而，在牛乳腺上皮细胞中它们之间的相关性仍不清楚。在此，确定AKT 1、STAT 5和mTOR的mRNA和蛋白质表达水平以及这些蛋白质的磷酸化。使用CASY-TT测定法检查细胞增殖和活力。将纯化的牛乳腺上皮细胞在分化培养基中培养不同时期。基本分化培养基含有胰岛素（5 μg/mL）、氢化可的松（1 μg/mL）和催乳素（5 μg/mL）的催乳激素混合物。在该培养基中培养的细胞生长缓慢，并且表达比对照细胞更高水平的p-STAT 5、p-AKT 1和p-mTOR（活化形式）。虽然磷酸化率没有增加，但这些蛋白质的转录和翻译通过添加胰岛素样生长因子-1或生长激素而上调，这进一步增加了β-酪蛋白mRNA的表达。此外，这三种蛋白的上调或下调同步，即使在RNA干扰沉默eitherStat 5或Akt 1。这些发现表明，一些激素和其他因素发挥催乳和催乳的作用，促进两个关键催乳信号与乳蛋白表达。我们提供了催乳素/STAT 5和AKT 1/mTOR同步化的证据，建立了牛乳腺上皮细胞中乳蛋白的转录调节和翻译调节之间的直接相关性。
The prolactin/STAT5 and AKT1/mTOR pathways play a key role in milk protein transcription and translation, respectively. However, the correlation between them in bovine mammary epithelial cells remains unclear. Here, mRNA and protein expression levels of AKT1, STAT5, and mTOR and the phosphorylation of these proteins were determined. Cell proliferation and viability were examined using the CASY-TT assay. Purified bovine mammary epithelial cells were cultured in differentiation media for different periods. The basic differentiation medium contained a lactogenic hormone cocktail of insulin (5 μg/mL), hydrocortisone (1 μg/mL), and prolactin (5 μg/mL). The cells cultured in this medium grew slowly and expressed higher levels of p-STAT5, p-AKT1, and p-mTOR (activated form) than those of control cells. Although the phosphorylation ratio was not increased, transcription and translation of these proteins were upregulated by the addition of insulin-like growth factor-1 or growth hormone, which further increasedβ-casein mRNA expression. Furthermore, the three proteins were upregulated or downregulated synchronously, even after RNA interference silencing of eitherStat5orAkt1. These findings indicate that a few hormones and other factors play lactogenic and galactogenic roles by promoting two key lactogenic signaling associated with milk protein expression. We provide evidence of prolactin/STAT5 and AKT1/mTOR synchronization, establishing a direct correlation between transcription regulation and translation regulation of milk protein in bovine mammary epithelial cells.
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影响因子：
3.5
作者：
Burgos, S. A.;Dai, M.;Cant, J. P.
通讯作者：
Cant, J. P.
影响因子：
5.3
作者：
Creamer, Bradley A.;Sakamoto, Kazuhito;Wagner, Kay-Uwe
通讯作者：
Wagner, Kay-Uwe
影响因子：
4.8
作者：
Wartmann, M;Cella, N;Hynes, NE
通讯作者：
Hynes, NE
影响因子：
4
作者：
Faraci-Orf, E;McFadden, C;Vogel, WF
通讯作者：
Vogel, WF
影响因子：
11.4
作者：
GOUILLEUX, F;WAKAO, H;GRONER, B
通讯作者：
GRONER, B