Role of iron and ethylenediaminetetraacetic acid in the bactericidal activity of a superoxide anion-generating system.
Role of iron and ethylenediaminetetraacetic acid in the bactericidal activity of a superoxide anion-generating system.
复制标题
铁和乙二胺四乙酸在超氧阴离子生成系统的杀菌活性中的作用。
DOI：
10.1016/0003-9861(81)90539-7
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发表时间：
1981
影响因子：
3.9
通讯作者：
Klebanoff,SJ
中科院分区：
文献类型：
--
作者：
Rosen,H;Klebanoff,SJ
在早期的研究中，发现由乙醛加黄嘌呤氧化酶组成的酶促超氧阴离子生成系统对金黄色葡萄球菌具有毒性。超氧阴离子（O）及其歧化产物过氧化氢（H2O2）都是必需的，并且提出（O）和H2O2相互作用形成更强大的杀菌剂，羟基自由基（OH·）和/或单线态氧。 EDTA 螯合的铁似乎是黄嘌呤氧化酶杀菌系统迄今为止未被认识到的要求。证据如下：（1）向黄嘌呤氧化酶系统添加铁盐可增加杀菌活性，而铁螯合剂二亚乙基三胺五乙酸（DTPA）和去铁胺具有抑制作用； (2)含EDTA的黄嘌呤氧化酶制剂的透析消除了杀菌活性，加入EDTA后又恢复了杀菌活性； (3) 通过使盐溶液通过 Chelex-100 柱去除痕量铁，消除了杀菌活性，而添加铁后又恢复了杀菌活性。铁和 EDTA 以 1:1 化学计量存在时最有效，并且它们不能被各种其他金属或螯合剂替代。乙醛-黄嘌呤氧化酶-铁-EDTA体系的杀菌活性被超氧化物歧化酶、过氧化氢酶以及OH·清除剂乙醇和甘露醇抑制，表明该复合物充当了(O)和H2O2之间形成OH·的反应的催化剂（Haber-Weiss反应）。考虑了铁-EDTA 相对催化特异性的可能原因。
In an earlier study, an enzymic superoxide anion-generating system consisting of acetaldehyde plus xanthine oxidase was found to be toxic toStaphylococcus aureus. Both superoxide anion (O) and its dismutation product hydrogen peroxide (H2O2) were required and it was proposed that (O) and H2O2interact to form the more powerful bactericidal agent(s), hydroxyl radical (OH·) and/or singlet oxygen. Iron chelated by EDTA appears to be a heretofore unrecognized requirement for the xanthine oxidase bactericidal system. The evidence is as follows: (1) the addition of iron salts to the xanthine oxidase system increased bactericidal activity whereas the iron chelators diethylenetriaminepentaacetic acid (DTPA) and desferrioxamine were inhibitory; (2) dialysis of the EDTA-containing xanthine oxidase preparation abolished bactericidal activity which was restored on the addition of EDTA; (3) removal of trace amounts of iron by passage of salt solutions through a Chelex-100 column abolished bactericidal activity which was restored on the addition of iron.Iron and EDTA were most effective when present at 1:1 stoichiometry and they could not be replaced by a variety of other metals or chelators. The bactericidal activity of the acetaldehyde-xanthine oxidase-iron-EDTA system was inhibited by superoxide dismutase, catalase, and the OH · scavengers ethanol and mannitol, suggesting that the complex served as a catalyst of the reaction between (O) and H2O2to form OH· (Haber-Weiss reaction). Possible reasons for the relative catalytic specificity of iron-EDTA are considered.