Role of folic acid in regulating gut microbiota and short-chain fatty acids based on an in vitro fermentation model
Role of folic acid in regulating gut microbiota and short-chain fatty acids based on an in vitro fermentation model
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DOI：
10.1007/s00253-023-12825-5
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发表时间：
2024-12-01
影响因子：
5
通讯作者：
Li，Ping
中科院分区：
文献类型：
--
作者：
Zheng，Xiaogu;Xia，Chenlan;Li，Ping
叶酸缺乏在世界范围内很常见，并与肠道微生物区系失衡有关。然而，基于用于研究肠道微生物利用叶酸的模型动物，存在重复性和个体差异的挑战。本研究建立了叶酸缺乏大鼠的体外粪便悬浮液培养模型，研究了添加5-甲基四氢叶酸(MTHF)和非还原叶酸(FA)对肠道微生物区系的调节作用。16S rRNA测序结果表明，与对照组(34.3%)相比，添加FA(29.7%)和MTHF(27.9%)均能显著降低类杆菌的相对丰度。添加MTHF可显著提高仔猪血清相对丰度4.49%。值得注意的是，与对照组相比，添加FA和MTHF促进了乳杆菌、双歧杆菌和小球菌粪便水平的增加。短链脂肪酸(SCFA)分析表明，补充叶酸降低了醋酸盐水平，增加了异丁酸的发酵产量。本研究建立的体外粪便悬浮液培养模型可以作为人体叶酸缺乏的模型，用于研究肠道微生物区系，证明外源叶酸影响肠道微生物区系组成和SCFA水平。关键：建立体外培养模型中叶酸缺乏的模型。·补充叶酸调节肠道微生物和SCFA。
Folic acid deficiency is common worldwide and is linked to an imbalance in gut microbiota. However, based on model animals used to study the utilization of folic acid by gut microbes, there are challenges of reproducibility and individual differences. In this study, an in vitro fecal slurry culture model of folic acid deficiency was established to investigate the effects of supplementation with 5-methyltetrahydrofolate (MTHF) and non-reduced folic acid (FA) on the modulation of gut microbiota. 16S rRNA sequencing results revealed that both FA (29.7%) and MTHF (27.9%) supplementation significantly reduced the relative abundance ofBacteroidetescompared with control case (34.3%). MTHF supplementation significantly improved the relative abundance ofFirmicutesby 4.49%. Notably, compared with the control case, FA and MTHF supplementation promoted an increase in fecal levels ofLactobacillus,Bifidobacterium, andPediococcus. Short-chain fatty acid (SCFA) analysis showed that folic acid supplementation decreased acetate levels and increased fermentative production of isobutyric acid. The in vitro fecal slurry culture model developed in this study can be utilized as a model of folic acid deficiency in humans to study the gut microbiota and demonstrate that exogenous folic acid affects the composition of the gut microbiota and the level of SCFAs.Key points•Establishment of folic acid deficiency in an in vitro culture model.•Folic acid supplementation regulates intestinal microbes and SCFAs.•Connections between microbes and SCFAs after adding folic acid are built.