Molecular phylogenetic analyses of snakeheads (Perciformes: Channidae) using mitochondrial DNA sequences
Molecular phylogenetic analyses of snakeheads (Perciformes: Channidae) using mitochondrial DNA sequences
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DOI：
10.1007/s10228-005-0321-3
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发表时间：
2006-05-01
影响因子：
1.2
通讯作者：
Kumazawa, Yoshinori
中科院分区：
文献类型：
--
作者：
Li, Xia;Musikasinthorn, Prachya;Kumazawa, Yoshinori
测定了淡水鱼科20种鱼类和南鳅科、斑鳅科和奥氏鳅科3种鱼类的线粒体DNA序列，包括NADH脱氢酶亚基2（ND 2）基因及其侧翼基因区，全长约1.5kbp。东方鳢（Channa orientalis）和东方鳢C. gachua在tRNA（Met）和ND 2基因之间具有大约170-bp的插入，其中5 ′-一半的插入与ND 2基因的5 ′-末端部分相似，3 ′-一半与tRNA（Met）基因同源。因此，这种插入可能起源于这两个姐妹物种的共同祖先中发生的串联基因重复。分子系统发育分析，从不同的树建设的方法一致建议的非洲和亚洲类群的相互单系性和存在的几个分支内的亚洲类群，其中一些对应于不同的形态特征。我们的分子遗传学清楚地支持了亚洲血统中多个独立的腹鳍的平行损失。在不假设分子钟的情况下，根据一些合理的假设估算了分歧时间，表明早白垩世非洲和亚洲的Channids发生了分歧。因此，这些结果支持了非洲和亚洲的Channids的古老的vicariant分歧，而不是最近的非洲和欧亚大陆之间的分散。
Mitochondrial DNA sequences of approximately 1.5 kbp including the NADH dehydrogenase subunit 2 (ND2) gene and its flanking gene regions were determined for 20 species from the freshwater fish family Channidae and 3 species from Nandidae, Badidae, and Osphronemidae. Channa orientalis and C. gachua had an approximately 170-bp insertion between the tRNA(Met) and ND2 genes, where a 5'-half of the insertion was similar to the 5'-end portion of the ND2 gene and a 3'-half was homologous to the tRNA(Met) gene. This insertion may thus have originated from a tandem gene duplication that occurred in a common ancestor of these two sister species. Molecular phylogenetic analyses from different tree-building methods consistently suggested the mutual monophyly of the African and Asian taxa and the existence of several clades within the Asian taxa, some of which correspond to distinct morphological features. Our molecular phylogeny clearly supported multiple independent losses of pelvic fins on Asian lineages in parallel. Divergence time estimation based on some reasonable assumptions without assuming the molecular clock suggested the early Cretaceous divergence of the African and Asian channids. The results thus support an ancient vicariant divergence of the African and Asian channids, rather than the more recent dispersal between African and Eurasian continents.