Deoxynucleoside triphosphate pool changes and UV-induced depression of DNA synthesis.
Deoxynucleoside triphosphate pool changes and UV-induced depression of DNA synthesis.
复制标题
脱氧核苷三磷酸库的变化和紫外线诱导的 DNA 合成抑制。
DOI：
10.1016/0006-291x(84)90292-4
复制
发表时间：
1984
影响因子：
3.1
通讯作者：
Ling,SY
中科院分区：
文献类型：
--
作者：
Griffiths,TD;Ling,SY
It has been reported that changes in deoxynucleotide pool levels following exposure to UV might lead to an overestimation of the UV-induced depression in DNA synthesis as analyzed by incorporation of3H-thymidine (1). We attempted to determine the importance of such pool effects in two ways. First, we examined the grain density along DNA fiber autoradiographs obtained from CHO AA8, and CHO UV-5 cells exposed or sham exposed to UV. Exposure to UV did not alter the grain density immediately or 5 hours after exposure to UV in these cell lines. Second, we examined the kinetics of incorporation of3H-dTTP in permeabilized AA8 cells under conditions of increased dCTP or increased exogenous dTTP levels. The extent of the depression of3H-dTTP was identical under all incubation conditions.
