Single-molecule detection and tracking in plants
Single-molecule detection and tracking in plants
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DOI：
10.1007/s00709-013-0601-0
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发表时间：
2014-01
期刊：
影响因子：
2.9
通讯作者：
M. Langhans;T. Meckel
中科院分区：
文献类型：
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作者：
M. Langhans;T. Meckel
将光学特性与有限的荧光团选择相结合，使植物中的单分子成像成为一项具有挑战性的任务。这就解释了为什么这项技术尽管在动物细胞生物学领域取得了成功，但远未被常规应用于植物细胞研究。然而，同样的挑战也适用于将单分子显微镜应用于任何完整组织或多细胞3D细胞培养。由于荧光显微镜最近和即将取得的进展将允许在多细胞系统的背景下进行单分子检测，植物组织成像将从这一进展中受益匪浅。在这篇综述中，我们讨论了单分子实验的每一步，强调了每一步的关键方面，并详细阐述了改进所需的优化和发展。我们涉及到最近的成就，这是迄今为止进行专门的根表皮ofArabidopsis thalianaseedlings倾斜照明，并显示使用不同的细胞或照明方案的单分子测量的例子。
Combining optical properties with a limited choice of fluorophores turns single-molecule imaging in plants into a challenging task. This explains why the technique, despite its success in the field of animal cell biology, is far from being routinely applied in plant cell research. The same challenges, however, also apply to the application of single-molecule microscopy to any intact tissue or multicellular 3D cell culture. As recent and upcoming progress in fluorescence microscopy will permit single-molecule detection in the context of multicellular systems, plant tissue imaging will experience a huge benefit from this progress. In this review, we address every step of a single-molecule experiment, highlight the critical aspects of each and elaborate on optimizations and developments required for improvements. We relate each step to recent achievements, which have so far been conducted exclusively on the root epidermis ofArabidopsis thalianaseedlings with inclined illumination and show examples of single-molecule measurements using different cells or illumination schemes.