Transcriptional Profiling Reveals Adaptive Responses to Boron Deficiency Stress in Arabidopsis
Transcriptional Profiling Reveals Adaptive Responses to Boron Deficiency Stress in Arabidopsis
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DOI：
10.5560/znc.2012.67c0510
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发表时间：
2012-09-01
影响因子：
2
通讯作者：
Xu, Fangsen
中科院分区：
文献类型：
--
作者：
Peng, Lishun;Zeng, Changying;Xu, Fangsen
硼（B）是维管植物的微量营养元素，B缺乏已被世界许多地区公认为是作物生产的限制因子。为了更好地了解植物对B饥饿的适应性机制，利用拟南芥全基因组Affyssin基因芯片研究了植物在短期和长期B缺乏胁迫下的基因表达变化。大量的B缺陷反应基因被鉴定并按其功能分组。与茉莉酸（JA）连锁的基因在B缺乏时表现出最显著的反应。在短期和长期的B缺乏胁迫过程中，JA的生物合成和调控转录本不断被诱导。一组已知的JA依赖的过程和响应基因显示出相同的表达谱。这表明，JA可能是一个关键的球员在整合的适应性反应B缺乏压力。此外，还观察到了其他B缺乏反应基因的功能群（包括各种编码抗氧化剂生物合成的基因、Ca ~（2+）信号的基本成分、蛋白激酶、细胞壁修饰酶和蛋白、H ~+-ATPase、K ~+转运蛋白以及一组参与中枢代谢和细胞生长的酶），并讨论了它们在B缺乏胁迫下的生理作用。这些结果为更好地理解植物对B缺乏胁迫的适应性反应和提高作物B效率的潜在策略提供了一些信息。
Boron (B) is a micronutrient for vascular plants, and B deficiency has been recognized as a limiting factor for crop production in many areas worldwide. To gain a better insight into the adaptability mechanism of plant responses to B starvation, an Arabidopsis whole genome Affymetrix Gene Chip was used to evaluate global gene expression alterations in response to short- and long-term B deficiency stress. A large number of B deficiency-responsive genes were identified and grouped by their functions. Genes linked to jasmonic acid (JA) showed the most prominent response under B deficiency. The transcripts for biosynthesis and regulation of JA were constantly induced during short- and long-term B deficiency stress. A set of well-known JA-dependent process and responsive genes showed the same expression profile. This suggested that JA could be a pivotal player in the integration of adaptive responses to B deficiency stress. Moreover, other functional groups of B deficiency-responsive genes (including various encoding the biosynthesis of antioxidants, the basic components of Ca2+ signalling, protein kinases, cell wall-modifying enzymes and proteins, H+-ATPase, K+ transporters, and a set of enzymes involved in central metabolism and cellular growth) were also observed, and their physiological roles under B deficiency stress are discussed. These results provide some information for a better understanding of plant-adaptive responses to B deficiency stress and potential strategies to improve B efficiency in crops.