An entomopoxvirus homologue of the vaccinia virus D13L-encoded 'rifampicin resistance' protein
An entomopoxvirus homologue of the vaccinia virus D13L-encoded 'rifampicin resistance' protein
复制标题
DOI：
10.1099/0022-1317-77-5-839
复制
发表时间：
1996-05-01
影响因子：
3.8
通讯作者：
Dall, DJ
中科院分区：
文献类型：
--
作者：
Osborne, RJ;Symonds, TM;Dall, DJ
棉铃虫昆虫痘病毒 (HaEPV) 基因组编码与痘苗病毒“利福平抗性”蛋白相关的预测 68 kDa 多肽（具有 30% 同一性）和同源猪痘病毒蛋白（27% 同一性）。我们无法分离出编码预测的蛋白质 C 端截短形式的 HaEPV 基因型变体，这表明该分子的 C 端可能对蛋白质功能至关重要，反过来，该功能可能对病毒复制至关重要。在暴露于利福平的宿主细胞中，HaEPV 复制大幅减少，但观察到该药物的细胞毒性特性使得无法确定这种抑制的具体原因。我们认为，拥有编码该多肽家族成员的基因可能代表了痘病毒科的定义性分子特征。
The Heliothis armigera entomopoxvirus (HaEPV) genome encodes a predicted 68 kDa polypeptide related to the 'rifampicin resistance' protein of vaccinia virus (with 30% identity), and an homologous swinepox virus protein (27% identity). We were unable to isolate an HaEPV genotypic variant encoding a predicted C-terminal truncated form of the protein, suggesting that the C terminus of the molecule may be essential to protein function, and, in turn, that this function may be essential to viral replication. HaEPV replication was substantially reduced in host cells exposed to rifampicin, but the observed cytotoxic properties of the the drug made it impossible to determine the specific cause of that inhibition. We suggest that possession of a gene encoding a member of this polypeptide family might represent a defining molecular characteristic of the Poxviridae.