Phosphoproteomics unveils stable energy supply as key to flooding tolerance in Kandelia candel.
Phosphoproteomics unveils stable energy supply as key to flooding tolerance in Kandelia candel.
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DOI：
10.1016/j.jprot.2018.01.008
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发表时间：
2018-03
影响因子：
3.3
通讯作者：
Dezhuo Pan;Lingxia Wang;Fanglin Tan;Si Lu;Xiaojie Lv;M. Zaynab;Chi-Lien Cheng;Y. Abubakar;
中科院分区：
文献类型：
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作者：
Dezhuo Pan;Lingxia Wang;Fanglin Tan;Si Lu;Xiaojie Lv;M. Zaynab;Chi-Lien Cheng;Y. Abubakar;
红树林秋茄Kandelia Candel(L.)德鲁斯每天都会经历洪水循环。探讨K的生理适应的分子机制。在烛光淹水过程中，通过使用等压标签进行相对和绝对定量的大规模磷酸蛋白质组分析，研究了蛋白质磷酸化在淹水反应中的潜在作用。从1516个磷酸蛋白墨汁中鉴定出2141个独特的磷酸化多肽和2603个非冗余的磷酸化位点。烛叶。除了已知的磷酸化基序外，还发现了三个新的基序[GSP]、[GxxSP]和[RSXS]。对96个差异表达的磷酸蛋白的磷酸化水平进行了鉴定，其中包括那些与丙酮酸代谢和能量产生有关的蛋白，以响应洪水。研究了与淹水反应相关的生理参数和转录水平，包括光合作用、丙酮酸代谢和ROS产生，发现它们在淹水条件下都是健壮的。磷蛋白组学、生理分析和转录水平的一致结果相互加强，证明K。蜡烛通过维持充足的光合作用活性、实现有效的无氧呼吸和增加戊糖磷酸途径的通量来适应淹水。蛋白质磷酸化可能在这些途径的调节中发挥主要作用，这些途径共同有助于稳定的能量供应，从而提高油墨的耐水泛性。淹水胁迫是主要的环境胁迫之一。在其自然栖息地，木质红树林Kandelia Candelia每天都会经历洪水循环。蛋白质磷酸化是植物对生物和非生物胁迫反应的重要调节机制。为了分析参与关键代谢途径的关键酶的磷酸化水平，我们采用了磷酸蛋白质组学的方法来剖析K的适应机制。烛光泛滥的条件。据我们所知，这是第一次对K进行大规模的定量磷酸蛋白质组分析。秋茄的洪水反应。采用多重iTRAQ定量蛋白质组学和纳米LC-MS/MS方法构建了磷酸化蛋白质组。我们的结果表明K。通过维持充足的光合作用活性、增强有效的无氧呼吸和增加磷酸戊糖途径(PPP)的通量，Candelis能够在淹水条件下获得稳定的能量供应。在光合作用、无氧呼吸和PPP中发现的蛋白质磷酸化可能在K的耐淹中起重要作用。秋茄。
The mangroveKandelia candel(L.) Druce experiences daily flooding cycles. To explore the molecular mechanism underlying the physiological adaptation ofK. candelto flooding, the potential role of protein phosphorylation in flooding responses was investigated by a large-scale quantitative phosphoproteomic analysis using isobaric tag for relative and absolute quantitation. Total 2141 unique phosphopeptides and 2603 non-redundant phosphorylation sites were identified from 1516 phosphoproteins inK. candelleaves. In addition to known phosphorylation motifs, three new motifs [GSP], [GxxSP] and [RSxS] were discovered. The phosphorylation levels of 96 differentially expressed phosphoproteins, including those involved in pyruvate metabolism and energy production, were identified in response to flooding. The physiological parameters and transcriptional levels relevant to flooding responses including photosynthesis, pyruvate metabolism, and ROS production were investigated and all were found to be robust under flooding conditions. The consistent results of the phosphoproteomic, physiological analyses and transcriptional levels reinforce each other to demonstrate thatK. candeladapts to flooding through maintaining sufficient photosynthesis activities, achieving effective anaerobic respiration and increasing pentose phosphate pathway flux. Protein phosphorylation is likely to play a major role in the regulation of these pathways which together contribute to stable energy supply that enhances flooding tolerance inK. candel.Biological significanceFlooding stress is one of the major environmental stresses. The woody mangroveKandelia candelexperiences daily flooding cycles in its natural habitat. Protein phosphorylation is a crucial regulatory mechanism in plants' responses to both biotic and abiotic stresses. To analyze phosphorylation levels in critical enzymes involved in key metabolic pathways, we employed phosphoproteomic approach to dissect the adaptive mechanism ofK. candelto flooding conditions. To our knowledge, this is the first large-scale quantitative phosphoproteomic analyses ofK. candel's flooding responses. Multiplex iTRAQ-based quantitative proteomic and Nano-LC–MS/MS methods were used to construct the phosphorproteome. Our results indicate thatK. candelis able to acquire stable energy supply under flooding by maintaining sufficient photosynthesis activities, enhancing effective anaerobic respiration and increasing pentose phosphate pathway (PPP) flux. The protein phosphorylation found in photosynthesis, anaerobic respiration and PPP is likely to play important roles in the flooding tolerance ofK. candel.