Antifungal activity of ibuprofen against aspergillus species and its interaction with common antifungal drugs
Antifungal activity of ibuprofen against aspergillus species and its interaction with common antifungal drugs
复制标题
布洛芬对曲霉菌的抗真菌活性及其与常用抗真菌药物的相互作用
DOI：
--
复制
发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Liu W
中科院分区：
文献类型：
--
作者：
Li L;Xu H;Chen W;Wan Zh;Li R;Liu W
背景：侵袭性曲霉病（IA）的发病率在多种疾病的免疫功能低下患者中增加。预后不良可能是由于治疗选择有限。本研究旨在评价布洛芬对临床分离的曲霉菌的抗真菌活性，以及它与唑类、两性霉素B或米卡芬净的相互作用。方法采用圆盘扩散法和标准肉汤微量稀释法检测布洛芬对10株烟曲霉、黄曲霉和地曲霉的抑菌活性。为测定布洛芬与伊曲康唑、伏立康唑、两性霉素B或米卡芬金联用对曲霉的交互作用，采用圆盘扩散法和棋盘法。结果圆盘扩散法在培养48 h时，布洛芬形成直径为（20.1±3.9）mm的生长抑制带；微量肉汤稀释法布洛芬对曲霉的最低抑菌浓度（MIC）为1000 ~ 2000 μg/ml，最低杀菌浓度（MFC）为2000 ~ 8000 μg/ml。5株菌株中有2株布洛芬与伊曲康唑或伏立康唑联用时生长抑制区大于单用。棋盘法测定结果显示，各菌株的分数抑制浓度指数（FICI）范围为1.125 ~ 2.500。结论布洛芬对曲霉有一定的抑制作用。布洛芬不影响伊曲康唑、伏立康唑、两性霉素B和米卡芬净对曲霉的体外抑菌活性。
Background The incidence of invasive aspergillosis (IA) has increased in frequency in immunocompromised patients with a variety of diseases. The poor prognosis might be due to limited treatment option. This study aimed to evaluate antifungal activity of ibuprofen against clinical isolates of aspergillus species, as well as its interaction with azoles or with amphotericin B or with micafungin. Methods Antifungal activity of ibuprofen against 10 strains of Aspergillus fumigatus, Aspergillus flavus, and Aspergillus terreus were tested with both disk diffusion assay and standard broth microdilution method. To determine whether ibuprofen combined with itraconazole, voriconazole, amphotericin B, or micafungin had interactive effects on aspergillus spp., we used both disk diffusion assay and Chequerboard method. Results As for disk diffusion method, ibuprofen produced a zone of growth inhibition with diameters of (20.1±3.9) mm at 48 hours of incubation. As for broth microdilution method, the minimal inhibitory concentration (MIC) ranges of ibuprofen against aspergillus spp. were 1000-2000 μg/ml, and the minimal fungicidal concentration (MFC) ranges of that was 2000-8000 μg/ml. For 2 of 5 isolates, when ibuprofen combined with itraconazole or voriconazole, the zones of growth inhibition were larger than those of the individual drug. The results of Chequerboard method showed that fractional inhibitory concentration index (FICI) ranges were 1.125-2.500. Conclusions Ibuprofen is active against aspergillus spp.. And ibuprofen does not affect the in vitro activity of itraconazole, voriconazole, amphotericin B or micafungin against aspergillus spp.