Development of an organ culture experimental system to investigate collagen synthesis in corpora cavernosa.
Development of an organ culture experimental system to investigate collagen synthesis in corpora cavernosa.
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开发器官培养实验系统来研究海绵体中胶原蛋白的合成。
DOI：
10.1038/sj.ijir.3900309
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Moreland,RB
中科院分区：
文献类型：
--
作者：
Newhall,PM;Traish,A;Moreland,RB
建立了一种家兔阴茎海绵体器官培养模型，以研究完整器官中纤维胶原的合成。将兔阴茎整块取出，切开阴茎海绵体，切成5 mm的小块，并在培养基中孵育24 h。通过（a）乳酸脱氢酶（LDH）释放、（B）蛋白质合成和（c）对外源性添加的细胞因子的反应能力评估组织活力和代谢及功能完整性。
An organ culture model of rabbit corpus cavernosum has been developed to investigate fibrillar collagen synthesis in intact organ. Rabbit peni were removed en bloc, the corpora cavernosa were dissected, sliced into 5 mm pieces and incubated in culture media up to 24 h. Tissue viability and metabolic and functional integrity were assessed by (a) lactate dehydrogenase (LDH) release,(b) protein synthesis, and (c) ability to respond to exogenously added cytokines.