Mapping and KASP marker development for wheat curl mite resistance in "TAM 112" wheat using linkage and association analysis
Mapping and KASP marker development for wheat curl mite resistance in "TAM 112" wheat using linkage and association analysis
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DOI：
10.1007/s11032-018-0879-x
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发表时间：
2018-10-01
影响因子：
3.1
通讯作者：
Liu, Shuyu
中科院分区：
文献类型：
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作者：
Dhakal, Smit;Tan, Chor-Tee;Liu, Shuyu
小麦卷曲螨（WCM, Aceria tosichella Keifer）和WCM传播的小麦条纹花叶病毒（WSMV， Tritimovirus属）是美国大平原小麦生产的破坏性制约因素。选育抗病小麦品种是减少小麦产量损失的一条经济可行的途径。本研究的目的是(a)分别利用90K Infinium iSelect SNP阵列和基因分型测序进行连锁和关联分析，鉴定小麦品种TAM 112 （PI 643143）对WCM抗性的紧密连锁标记，以及(b)开发和测试用于WCM抗性标记辅助选择（MAS）的竞争性等位基因特异性PCR （KASP）单核苷酸多态性（SNP）。对124个由TAM 112与wcm敏感品种TAM 111 （PI 631352）杂交而成的F-5:7重组自交系（RILs）进行了试验。所有品系都被一种得克萨斯WCM 2 （TWCMC2）侵染，该菌株对小麦-黑麦1AL上发现的抗性具有毒性。在侵染后的第1周和第2周，对侵染后的症状进行评分。利用覆盖21条染色体的4890个标记构建连锁图谱，包括snp、简单序列重复（SSRs）和序列标记位点（STS）标记。将TAM112中存在的抗WCM基因（Cmc(TAM112)）定位到染色体臂6DS上。一项来自科罗拉多州的小麦条纹花叶病（WSM）症状的全基因组关联研究显示，在6DS上Cmc（TAM112）所在的靶区域存在显著的标记-性状关联，这证明了该基因在减轻症状严重程度方面的有效性。在双亲本定位群体中，在3.6cM范围内定位到Cmc（TAM112）侧的4个snp。我们开发了两个位于Cmc远端1.3cM以内的KASP标记（TAM112），并在不同的种质中进行了测试。在某些遗传背景下，这两个标记可用于MAS中提高小麦抗WCM能力。
Wheat curl mite (WCM, Aceria tosichella Keifer) and WCM-transmitted wheat streak mosaic virus (WSMV, genus Tritimovirus) are devastating production constraints for wheat in the US Great Plains. Breeding wheat cultivars with genetic resistance to WCM and WSMV is a viable and economically feasible way to reduce yield loss. The objectives of this study were to (a) identify tightly linked markers for WCM resistance in the wheat cultivar TAM 112 (PI 643143) using linkage and association analysis with the 90K Infinium iSelect SNP array and genotyping-by-sequencing, respectively and (b) develop and test kompetitive allele specific PCR (KASP) single-nucleotide polymorphisms (SNPs) for marker-assisted selection (MAS) of WCM resistance. We tested 124 F-5:7 recombinant inbred lines (RILs) derived from the cross of TAM 112 and the WCM-susceptible cultivar TAM 111 (PI 631352). All lines were infested with a Texas WCM collection 2 (TWCMC2) that is virulent to resistance found on the wheat-rye 1AL.1RS translocation at the two-leaf stage and were rated for symptoms on the first and second week after infestation. Linkage maps were constructed with 4890 markers, including SNPs, simple sequence repeats (SSRs), and sequence-tagged site (STS) markers covering 21 chromosomes. A WCM resistance gene present in TAM 112 (Cmc(TAM112)) was mapped onto chromosome arm 6DS. A genome-wide association study of wheat streak mosaic (WSM) symptoms from a separate experiment in Colorado showed significant marker-trait associations at the target regions on 6DS where Cmc(TAM112) was located, which demonstrated the effectiveness of this gene to reduce symptom severity. Four SNPs flanking Cmc(TAM112) were mapped within 3.6cM in the biparental mapping population. We developed two KASP markers that are within 1.3cM distal to Cmc(TAM112) and tested in diverse germplasm. These two markers can be used in MAS for improving WCM resistance in some wheat genetic backgrounds.