Sulfohydrolytic degradation of 3'-phosphoadenosine 5'-phosphosulfate (PAPS) and adenosine 5'-phosphosulfate (APS) by enzymes of a nucleotide pyrophosphatase nature.
Sulfohydrolytic degradation of 3'-phosphoadenosine 5'-phosphosulfate (PAPS) and adenosine 5'-phosphosulfate (APS) by enzymes of a nucleotide pyrophosphatase nature.
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通过核苷酸焦磷酸酶性质的酶对 3-磷酸腺苷 5-磷酸硫酸盐 (PAPS) 和腺苷 5-磷酸硫酸盐 (APS) 进行磺基水解降解。
DOI：
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发表时间：
1981
期刊：
影响因子：
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通讯作者：
I. Yamashina
中科院分区：
文献类型：
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作者：
S. Fukui;H. Yoshida;I. Yamashina
发现在大鼠的各种组织中广泛分布有降解活性硫酸盐（3 '-磷酸腺苷5'-磷酸硫酸盐，PAPS）及其前体APS（腺苷5 '-磷酸硫酸盐）的磺基水解活性，其最适pH为9.5。在肝脏中，活性位于质膜和内质网。TritonX-100溶解的粗面内质网和光面内质网在凝胶过滤上有两个活性峰，这两个活性峰都具有核苷酸焦磷酸酶活性，除了水解PAPS和APS外，还水解ATP、NAD和UDP-Glc的焦磷酸键和PNTP（p-nitrophenyl-tymidine 5 '-monophosphate）的磷酸二酯键。
The sulfohydrolytic activity to degrade active sulfate (3'-phosphoadenosine 5'-phosphosulfate, PAPS) and its precursor, APS (adenosine 5'-phosphosulfate), with a pH optimum at 9.5 was found to be widely distributed in various tissues of rats. In the liver, the activity was located in plasma membranes and endoplasmic reticula. Triton X-100 solubilized rough and smooth endoplasmic reticula gave two peaks of the activity on gel filtration, both of which had nucleotide pyrophosphatase activities, hydrolyzing the pyrophosphate linkages of ATP, NAD, and UDP-Glc, and the phosphodiester linkage of PNTP (p-nitrophenyl-thymidine 5'-monophosphate) besides PAPS and APS.