Molecular profiling of rhizosphere microbial communities associated with healthy and diseased black spruce (Picea mariana) seedlings grown in a nursery
Molecular profiling of rhizosphere microbial communities associated with healthy and diseased black spruce (Picea mariana) seedlings grown in a nursery
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DOI：
10.1128/aem.70.6.3541-3551.2004
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发表时间：
2004-06-01
影响因子：
4.4
通讯作者：
St-Arnaud, M
中科院分区：
文献类型：
--
作者：
Filion, M;Hamelin, RC;St-Arnaud, M
通过克隆 rRNA 基因序列分析，对健康黑云杉 (Picea mariana) 幼苗和有根腐病症状的幼苗根际相关的细菌和真菌种群进行了表征。构建了三份细菌和真菌 rRNA 基因文库，并通过扩增核糖体 DNA 限制性分析对 600 个克隆进行了分析，并将其分组为操作分类单位 (OTU)。总共获得了 84 个不同的细菌和 31 个不同的真菌 OTU 并进行了测序。系统发育分析表明不同的OTU属于多种细菌和真菌类群。对于两组，成对比较显示，每个处理的重复文库之间的相似性高于不同处理的文库之间的相似性。对于细菌和真菌，来自健康幼苗基因文库的一式三份合并样本与患病幼苗基因文库中的一式三份合并样本之间也存在显着差异，这清楚地表明健康和患病 P. mariana 幼苗的根际相关细菌和真菌群落是不同的。与健康和患病幼苗相关的群落也表现出不同的生态参数，如计算的多样性、优势度和均匀度指数所示。在群落水平上观察到的主要差异中，与健康幼苗相关的酸杆菌、γ-变形菌和同担子菌克隆的比例较高，而患病幼苗根际的放线菌、梭状菌和环境克隆的比例较高。这项研究中描述的方法似乎有望针对针叶树苗圃中发生的根腐病针对潜在的根际有效生物防治剂。
Bacterial and fungal populations associated with the rhizosphere of healthy black spruce (Picea mariana) seedlings and seedlings with symptoms of root rot were characterized by cloned rRNA gene sequence analysis. Triplicate bacterial and fungal rRNA gene libraries were constructed, and 600 clones were analyzed by amplified ribosomal DNA restriction analysis and grouped into operational taxonomical units (OTUs). A total of 84 different bacterial and 31 different fungal OTUs were obtained and sequenced. Phylogenetic analyses indicated that the different OTUs belonged to a wide range of bacterial and fungal taxa. For both groups, pairwise comparisons revealed that there was greater similarity between replicate libraries from each treatment than between libraries from different treatments. Significant differences between pooled triplicate samples from libraries of genes from healthy seedlings and pooled triplicate samples from libraries of genes from diseased seedlings were also obtained for both bacteria and fungi, clearly indicating that the rhizospher-eassociated bacterial and fungal communities of healthy and diseased P. mariana seedlings were different. The communities associated with healthy and diseased seedlings also showed distinct ecological parameters as indicated by the calculated diversity, dominance, and evenness indices. Among the main differences observed at the community level, there was a higher proportion of Acidobacteria, Gammaproteobacteria, and Homobasidiomycetes clones associated with healthy seedlings, while the diseased-seedling rhizosphere harbored a higher proportion of Actinobacteria, Sordariomycetes, and environmental clones. The methodological approach described in this study appears promising for targeting potential rhizosphere-competent biological control agents against root rot diseases occurring in conifer nurseries.