Metabolome analysis of Escherichia coli ATCC25922 cells treated with high hydrostatic pressure at 400 and 600 MPa
Metabolome analysis of Escherichia coli ATCC25922 cells treated with high hydrostatic pressure at 400 and 600 MPa
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DOI：
10.1016/j.jbiosc.2018.05.007
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发表时间：
2018-11-01
影响因子：
2.8
通讯作者：
Yamamoto, Kazutaka
中科院分区：
文献类型：
--
作者：
Kimura, Keitarou;Inaoka, Takashi;Yamamoto, Kazutaka
用400和600 MPa的高静水压（HHP）处理大肠杆菌细胞。使用毛细管电泳飞行时间质谱法分析从HHP处理的细胞中提取的金属离子（70-1027 m/z），并与从对照细胞（未用HHP处理）中提取的金属离子进行比较。共鉴定了133种代谢物并映射到代谢途径，其中许多（42.1%）由于HHP处理而减少，包括NAD（+），NADP（+），ATP和DNA合成底物。主成分分析表明，HHP对糖和核酸代谢途径有显著影响。在HHP处理的细胞中观察到中央糖代谢途径中的瓶颈，这造成了代谢失衡;上游映射的代谢物（葡萄糖6磷酸、果糖6磷酸和果糖1，6-二磷酸）积累，而下游的代谢物（3-磷酸甘油酸、2-磷酸甘油酸和磷酸烯醇丙酮酸）耗尽。核糖核苷酸减少，但减少是温和的相比，DNA合成的底物，例外的是ATP，这也大大减少。糖酵解途径中的瓶颈部分解释了ATP的耗尽。HHP处理细胞的NAD（+）/NADH比值与未处理的对照细胞相当。(C)2018年，日本生物技术学会。All rights reserved.
Escherichia coli cells were treated with high hydrostatic pressure (HHP) at 400 and 600 MPa. Metabolites (70-1027 m/z) extracted from HHP-treated cells were analyzed using capillary electrophoresis time-of-flight mass spectrometry and were compared with those extracted from control cells (not treated with HHP). A total of 133 metabolites were identified and mapped to metabolic pathways, and many of these (42.1%) decreased due to the HHP treatment, including NAD(+), NADP(+), ATP, and substrates for DNA synthesis. Principal component analysis suggested that the central sugar and nucleic acid metabolic pathways were strongly influenced by HHP. A bottleneck in the central sugar metabolic pathway was observed in HHP-treated cells, which created a metabolic imbalance; metabolites mapped upstream (glucose 6 phosphate, fructose 6-phosphate, and fructose 1,6-diphosphate) were accumulated and those downstream (3-phosphoglycerate, 2-phosphoglycerate, and phosphoenolpyruvate) were depleted. Ribonucleotides were decreased, but the reduction was moderate compared with that of substrates for DNA synthesis; the exception was ATP, which also substantially decreased. The bottleneck in the glycolytic pathway partly explained the exhaustion of ATP. NAD(+)/NADH ratio of HHP treated cells was comparable with that of untreated control cells. (C) 2018, The Society for Biotechnology, Japan. All rights reserved.