IL-13 pre-treatment of murine peritoneal macrophages increases their anti-Toxoplasma gondii activity induced by lipopolysaccharides
IL-13 pre-treatment of murine peritoneal macrophages increases their anti-Toxoplasma gondii activity induced by lipopolysaccharides
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DOI：
10.1016/j.ijpara.2007.08.002
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发表时间：
2008-03-01
影响因子：
4
通讯作者：
Pipy, Bernard
中科院分区：
文献类型：
--
作者：
Authier, Helene;Cassaing, Sophie;Pipy, Bernard
Th1细胞因子和微生物脂多糖（LPS）激活巨噬细胞产生炎症介质和效应分子。虽然Th2细胞因子通常具有与Th1细胞因子相反的作用并下调巨噬细胞的炎症反应，但它们可以诱导一种独特的替代激活，这有利于宿主防御。在这项研究中，我们报道了IL-13增强了lps激活的小鼠巨噬细胞抗弓形虫的活性。对寄生虫增殖的抑制与弓形虫侵入细胞的减少无关，也与速殖子向慢殖子的转化无关。单独使用时，IL-13可触发巨噬细胞向2型分化。然而，在lps激活的巨噬细胞中，我们发现这种细胞因子能够增强诱导型一氧化氮合酶（iNOS）的表达，iNOS是I型巨噬细胞的主要标志物。IL-13的这种作用不依赖于巨噬细胞的激活状态（常驻与巯基乙酸诱导）或预处理的时间。我们证明了增强NO生产和巨噬细胞杀微生物有效性升级之间的相关性。因此，Th2和Th1细胞因子都可以激活巨噬细胞来控制感染。(c) 2007年澳大利亚寄生虫学学会Elsevier Ltd.出版。版权所有。
Th1 cytokines and microbial lipopolysaccharides (LPS) activate macrophages to produce inflammatory mediators and effector molecules. Althrough Th2 cytokines often have an opposite action to Th1 cytokines and down-modulate the inflammatory response of macrophages, they can induce a distinct alternative activation that is beneficial in host defence. In this study, we report that IL-13 enhances the anti-Toxoplasma activity of LPS-activated murine macrophages. The inhibition of parasite proliferation was not related to reduced Toxoplasma gondii penetration into the cells, nor to the conversion of tachyzoites into bradyzoites. Used alone, IL-13 triggers the polarisation of macrophages towards type 2. However, in LPS-activated macrophages, we show the priming capacity of this cytokine to enhance the expression of inducible nitric oxide synthase (iNOS), a major marker of, type I macrophages. This effect of IL-13 was not dependent on the activation state of macrophages (resident versus thioglycolate-elicited) or the timing of pre-treatment. We demonstrate a correlation between the enhancement of NO production and upgrading of the microbicidal effectiveness of the macrophages. Thus, both Th2 and Th1 cytokines could activate macrophages to control infections. (c) 2007 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.