Cholesterol and fat in diet disrupt bone and tooth homeostasis in mice
Cholesterol and fat in diet disrupt bone and tooth homeostasis in mice
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饮食中的胆固醇和脂肪会破坏小鼠的骨骼和牙齿稳态
DOI：
10.1016/j.biopha.2022.113940
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发表时间：
2022
期刊：
影响因子：
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通讯作者：
Sakai Nobuhiro
中科院分区：
文献类型：
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作者：
Sato Yurie;Hosonuma Masahiro;Sugawara Daiki;Azetsu Yuki;Karakawa Akiko;Chatani Masahiro;Funatsu Takahiro;Takami Masamichi;Sakai Nobuhiro
血脂异常是血液中甘油三酯和胆固醇含量高的一种病症，而高胆固醇含量与多种全身性疾病有关。高脂肪饮食对骨骼的影响已有报道，然而，尚不清楚高脂肪饮食的哪些成分会影响骨骼。这项研究的目的是检查膳食脂质和胆固醇对骨稳态维持的影响。八周大的雄性小鼠 (C57BL/6 J) 被喂食五种不同含量的脂肪 (14%、36%) 和胆固醇 (0.01%、1.25%、5%) 的饲料，为期 12 周。检查血液、股骨、胫骨和牙齿样本，并使用 µCT 和组织学分析确定血清脂质标记物和骨形态。此外，获得并培养骨髓细胞，并使用 qPCR 分析破骨细胞分化标记物。与标准饮食组相比，喂食高脂肪（36%）和高胆固醇（1.25%）饮食的小鼠血液中总胆固醇和低密度脂蛋白水平升高，骨体积分数降低。然而，仅高脂肪组（36%）和仅高胆固醇组（1.25%、5%）的骨量不受影响。给予高脂肪（36%）饮食的小鼠也表现出门牙明显变窄。相反，各组之间的破骨细胞形成没有显着差异。这些结果表明，含有大量脂肪和胆固醇的饮食会导致骨质流失。
Dyslipidemia is a condition of high levels of triglycerides and cholesterol in the blood, and high levels of cholesterol is associated with a variety of systemic diseases. The effects of a high-fat diet on bone have been reported, however, it is not clear which components of a high-fat diet affect bone. This study was conducted to examine the effects of dietary lipids and cholesterol on bone homeostasis maintenance. Eight-week-old male mice (C57BL/6 J) were fed five types of feed with different amounts of fat (14 %, 36 %) and cholesterol (0.01 %, 1.25 %, 5 %) for 12 weeks. Blood, femur, tibia, and tooth samples were examined, and serum lipid markers and bone morphology were determined using µCT and histological analysis. Additionally, bone marrow cells were obtained and cultured, and osteoclast differentiation markers analyzed using qPCR. Mice fed a diet high in both fat (36 %) and cholesterol (1.25 %) showed increased total cholesterol and low-density lipoprotein levels in blood, and decreased bone volume fraction as compared to the standard diet group. However, bone mass was unaffected in the high fat only (36 %) and high cholesterol only (1.25 %, 5 %) groups. Mice given a high fat (36%) diet also demonstrated significantly narrowed incisor pulp. In contrast, osteoclast formation was not significantly different among the groups. These results suggest that a diet with high amounts of both fat and cholesterol induces bone loss.