RATE OF RELEASE OF EXTRACELLULAR AMINO-ACIDS AND CARBOHYDRATES FROM THE MARINE DIATOM CHAETOCEROS-AFFINIS
RATE OF RELEASE OF EXTRACELLULAR AMINO-ACIDS AND CARBOHYDRATES FROM THE MARINE DIATOM CHAETOCEROS-AFFINIS
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DOI：
10.1093/plankt/11.4.763
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发表时间：
1989-07-01
影响因子：
2.1
通讯作者：
VOLCANI, BE
中科院分区：
文献类型：
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作者：
MYKLESTAD, S;HOLMHANSEN, O;VOLCANI, BE
在分批培养条件下研究了海洋硅藻Chaetoceros affinis的排泄物。快速生长期细胞的碳水化合物和氨基酸的释放速率高于静止期细胞。然而，由于每个细胞的光合作用显着下降，在营养耗尽，排泄构成58%的总光合作用在静止的细胞相比，在指数增长的10%。在指数期最突出的胞外氨基酸是天冬氨酸、谷氨酸、丝氨酸、谷氨酰胺、甘氨酸、丙氨酸、缬氨酸和亮氨酸。在稳定期也产生精氨酸、天冬酰胺、酪氨酸和异亮氨酸。碳水化合物是释放量最大的胞外组分，其中多糖占80%以上。
Excretion from the marine diatom Chaetoceros affinis was investigated in batch cultures. The rates of release of carbohydrates and amino acids per cell were higher in rapidly growing cells than in stationarly phase cells. However, because photosynthesis per cell decreased significantly during nutrient depletion, excretion constituted 58% of total photosynthesis in stationary cells compared to 10% during exponential growth. The most prominent extracellular amino acids in the exponential phase were aspartic acid, glutamic acid, serine, glutamine, glycine, alanine, valine and leucine. In the stationary phase arginine, asparagine, tryrosine and isoleucine were also produced. Carbohydrate, of which polysaccharide constituted > 80%, was the most abundant extracellular component released.