Diagnostic and co-dominant PCR markers for wheat stem rust resistance genes Sr25 and Sr26
Diagnostic and co-dominant PCR markers for wheat stem rust resistance genes Sr25 and Sr26
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DOI：
10.1007/s00122-009-1186-z
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发表时间：
2010-02-01
影响因子：
5.4
通讯作者：
Anderson, James A.
中科院分区：
文献类型：
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作者：
Liu, Sixin;Yu, Long-Xi;Anderson, James A.
小麦秆锈病是由禾柄锈菌（Puccinia graminis f.）小麦赤霉病是小麦最具破坏性的病害之一。一个新的小种命名为TTKSK（syn. Ug 99）及其衍生物对许多指定和未指定的茎锈病抗性基因具有毒性。这些小种的出现和传播对全世界小麦生产构成了迫在眉睫的威胁。从Thinopyrum ponticum转入小麦的基因Sr 25和Sr 26对这些新小种有效。需要Sr 25和Sr 26的DNA标记来将这两个基因聚合成适应的种质。用8个含有或不含有Sr 25或Sr 26的小麦品系验证了先前发表的Sr 25的显性标记Gb和Sr 26的显性标记Sr26#43。我们测试了六个已发表的STS（序列标记位点）标记扩增Th的诊断带。ponticum。标记BF 145935一直扩增良好，可用作Sr 25的共显性标记。在定位于6AL 8 -0.90-1.00的小麦EST序列中，没有一个STS标记是共显性的。然而，鉴定了5个6A特异性标记。具有标记Sr26#43和6A特异性标记BE 518379的多重PCR可用作Sr 26的共显性标记。利用37个已知抗秆锈基因的株系对Sr 25和Sr 26的共显性标记进行了验证。利用Sr 25和Sr 26的共显性标记对来自CIMMYT、中国、美国等国家的170份小麦种质资源进行了筛选。鉴定了具有Sr 25的诊断片段的五个品系，并且它们在它们的谱系中都具有已知携带Sr 25的“Wheatear”。正如预期的那样，测试的170个品系中没有一个含有Sr 26。
Wheat stem rust, caused by Puccinia graminis f. sp. tritici, is one of the most destructive diseases of wheat. A new race of the pathogen named TTKSK (syn. Ug99) and its derivatives detected in East Africa are virulent to many designated and undesignated stem rust resistance genes. The emergence and spread of those races pose an imminent threat to wheat production worldwide. Genes Sr25 and Sr26 transferred into wheat from Thinopyrum ponticum are effective against these new races. DNA markers for Sr25 and Sr26 are needed to pyramid both genes into adapted germplasm. The previously published dominant markers Gb for Sr25 and Sr26#43 for Sr26 were validated with eight wheat lines with or without Sr25 or Sr26. We tested six published STS (sequence tagged site) markers amplifying diagnostic bands of Th. ponticum. Marker BF145935 consistently amplified well and can be used as a co-dominant marker for Sr25. Among 16 STS markers developed from wheat ESTs mapped to deletion bin 6AL8-0.90-1.00, none was co-dominant for tagging Sr26. However, five 6A-specific markers were identified. Multiplex PCR with marker Sr26#43 and 6A-specific marker BE518379 can be used as a co-dominant marker for Sr26. The co-dominant markers for Sr25 and Sr26 were validated with 37 lines with known stem rust resistance genes. A diverse set of germplasm consisting 170 lines from CIMMYT, China, USA and other counties were screened with the co-dominant markers for Sr25 and Sr26. Five lines with the diagnostic fragment for Sr25 were identified, and they all have 'Wheatear' in their pedigrees, which is known to carry Sr25. None of the 170 lines tested had Sr26, as expected.