Optimal testing conditions for determining MICs and minimum fungicidal concentrations of new and established antifungal agents for uncommon molds: NCCLS collaborative study
Optimal testing conditions for determining MICs and minimum fungicidal concentrations of new and established antifungal agents for uncommon molds: NCCLS collaborative study
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DOI：
10.1128/jcm.40.10.3776-3781.2002
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发表时间：
2002-10-01
影响因子：
9.4
通讯作者：
Rinaldi, MG
中科院分区：
文献类型：
--
作者：
Espinel-Ingroff, A;Chaturvedi, V;Rinaldi, MG
本三中心协作研究评价了NCCLS M38-A文件检测条件和其他检测条件对35株构巢曲霉、A. terreus，夏威夷双脐蠕孢（Bipolaris hawaiiensis），B. spicifiera、Cladophialophora bantiana、Dactylaria constricta、Fusarium solani、Paecilomyces lilacinus、Scedosporium prolificans、Trichoderma longibrachiatum和Wangiella dermativelum对伊曲康唑、三种新的三唑（伏立康唑、泊沙康唑和拉夫康唑）和阿替霉素B的作用。使用4种培养基测定每个中心的MIC和最低杀真菌浓度（MFC（标准RPMI-1640 [RPMI]、含2%葡萄糖的RPMI [RPMI-2%]、抗生素培养基3 [M3]和含2%葡萄糖的M3 [M3-2%]）和两个MIC测定标准（完全生长抑制[MIC-0]和显著生长抑制[MIC-2]）。MFC被定义为最低药物浓度，
This collaborative three-center study evaluated NCCLS M38-A document testing conditions and other testing conditions for the antifungal susceptibility testing of 35 isolates of Aspergillus nidulans, A. terreus, Bipolaris hawaiiensis, B. spicifiera, Cladophialophora bantiana, Dactylaria constricta, Fusarium solani, Paecilomyces lilacinus, Scedosporium prolificans, Trichoderma longibrachiatum, and Wangiella dermatitidis for itraconazole, three new triazoles (voriconazole, posaconazole, and ravuconazole), and amphotericin B. MICs and minimum fungicidal concentrations (MFCs) were determined in each center by using four media (standard RPMI-1640 [RPMI], RPMI with 2% dextrose [RPMI-2%], antibiotic medium 3 [M3], and M3 with 2% dextrose [M3-2%]) and two criteria of MIC determination (complete growth inhibition [MICs-0] and prominent growth inhibition [MICs-2]) at 24, 48 and 72 h. MFCs were defined as the lowest drug concentrations that yielded