Determination of Reactive Carbonyl Species, Which Mediate Reactive Oxygen Species Signals in Plant Cells
Determination of Reactive Carbonyl Species, Which Mediate Reactive Oxygen Species Signals in Plant Cells
复制标题
植物细胞中介导活性氧信号的活性羰基物种的测定
DOI：
10.1007/978-1-0716-2469-2_15
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发表时间：
2022
影响因子：
--
通讯作者：
Murata Yoshiyuki
中科院分区：
文献类型：
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作者：
Mano Jun’ichi;Biswas Md. Sanaullah;Sugimoto Koichi;Murata Yoshiyuki
植物细胞对活性氧（ROS）的反应，例如，防御基因的重编程或细胞死亡的进展，应该包括ROS信号传递到靶蛋白，但这个过程的生物化学在很大程度上是未知的。脂质过氧化物衍生的α，β-不饱和醛和酮（反应性羰基物质; RCS）是ROS刺激的下游产物，是最近出现的内源性物质，可以通过共价修饰介导ROS信号到蛋白质。RCS参与某些ROS信号在植物中（氧化损伤的叶和根，ROS诱导的程序性细胞死亡，衰老，脱落酸和生长素信号）已被验证的RCS与使用常规的HPLC测定。由于不同种类的RCS在细胞中的作用不同，因此被代谢，植物组织中每个RCS的鉴定和定量提供了核心信息，以破译植物对ROS反应的生化机制。本文介绍了植物样品制备、RCS提取和分析的实用方法。
Responses of plant cells to reactive oxygen species (ROS), e.g., reprogramming of defense genes or progression of cell death, should include the ROS signal transmission to target proteins, but the biochemistry of this process is largely unknown. Lipid peroxide-derivedα,β-unsaturated aldehydes and ketones (reactive carbonyl species; RCS), downstream products of ROS stimuli, are recently emerging endogenous agents that can mediate ROS signal to proteins via covalent modification. The involvement of RCS in certain ROS signaling in plants (oxidative injury of leaves and roots, ROS-induced programmed cell death, senescence, and abscisic acid and auxin signaling) has been verified by the determination of RCS with the use of conventional HPLC. Because distinct kinds of RCS act differently in the cell and so are metabolized, identification and quantification of each RCS in plant tissues provide central information to decipher biochemical mechanisms of plant responses to ROS. This article illustrates practical methods of plant sample preparation and extraction and analysis of RCS.