Characterization of a region of plasmid pBL1 of Brevibacterium lactofermentum involved in replication via the rolling circle model
Characterization of a region of plasmid pBL1 of Brevibacterium lactofermentum involved in replication via the rolling circle model
复制标题
通过滚环模型表征乳发酵短杆菌质粒 pBL1 参与复制的区域
作者：
C. Fernández;R. F. Cadenas;M. Noirot;J. Martín;J. Gil
pBL1 是一种 4.5 kb 的乳发酵短杆菌 ATCC 13869 隐性质粒，已用于构建多种棒状杆菌载体，其自主复制的最小区域被证明包含在 1.8 kb HindII-SphI DNA 片段上。该区域包含两个开放阅读框 (ORF)（ORF1 和 ORF5），它们对于乳发酵双歧杆菌中的 pBL1 复制至关重要。一些构建体中单链中间体的积累表明质粒 pBL1 通过滚环复制模型进行复制；通过与与每条pBL1链互补的两个合成寡核苷酸探针杂交来鉴定其正链和负链。 ORF1 似乎编码 Rep 蛋白，并且与链霉菌属质粒（例如 pIJ101、pSB24 和 pJV1）中通过滚环复制进行复制的 Rep 蛋白序列显示出部分同源性。
The minimal region for autonomous replication of pBL1, a 4.5-kb cryptic plasmid of Brevibacterium lactofermentum ATCC 13869 that has been used to construct a variety of corynebacterium vectors, was shown to be contained on a 1.8-kb HindII-SphI DNA fragment. This region contains two open reading frames (ORFs) (ORF1 and ORF5) which are essential for pBL1 replication in B. lactofermentum. Accumulation of single-strand intermediates in some of the constructions indicates that plasmid pBL1 replicates via the rolling circle replication model; its plus strand and minus strand were identified by hybridization with two synthetic oligonucleotide probes complementary to each pBL1 strand. ORF1 seems to encode the Rep protein and showed partial homology with sequences for Rep proteins from Streptomyces plasmids which replicate via rolling circle replication such as pIJ101, pSB24, and pJV1.
葡萄球菌质粒及其复制。
影响因子：
10.5
作者：
Novick,RP
通讯作者：
Novick,RP