Male-female interaction and temperature variation affect pollen performance in Citrus
Male-female interaction and temperature variation affect pollen performance in Citrus
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DOI：
10.1016/j.scienta.2012.03.011
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发表时间：
2012-06-01
影响因子：
4.3
通讯作者：
Gentile, Alessandra
中科院分区：
文献类型：
--
作者：
Distefano, Gaetano;Hedhly, Afif;Gentile, Alessandra
尽管对几种植物物种的花粉性能进行了广泛的研究工作，但在一些经济上重要的物种（如柑橘）中对其花粉性能进行了较少的研究，在柑橘中，有性生殖过程的失败以及随后的单性结实果实发育和无籽是重要的特征。在这项工作中，我们表征了三个祖先真实型柑橘属物种的花粉-雌蕊相互作用，以确定雄性和雌性基因型以及温度状况对花粉性能的影响。具体来说，我们表征了田间温度条件下温度对体外花粉粒萌发以及对切花和植物体内花粉管生长的影响。获得的结果表明，温度变化对花粉萌发、花粉管动力学及其种群大小（取决于特定的雌雄组合）有很大影响。体外花粉萌发的最适温度为 25 摄氏度，而加速体内花粉管生长的最有利温度取决于特定的雌雄相互作用，范围在 15 至 25 摄氏度之间。此外，温度似乎通过影响花粉管停滞的位置来影响自交不亲和反应。总的来说，我们的结果表明，花粉性能不仅是花粉基因型的固有特征，而且在很大程度上取决于特定的雄性-雌性组合以及基因型-温度相互作用。 (C) 2012 Elsevier B.V. 保留所有权利。
Despite the extensive research work characterizing pollen performance in several plant species, less effort has been made to characterize it in some economically important species as Citrus, in which the failure of the sexual reproductive process, and subsequent parthenocarpic fruit development and seedlessness are prized characters. In this work we characterized pollen-pistil interaction in the three ancestral true-type Citrus species in order to determine the influence of the male and female genotypes, as well as of the temperature regime on pollen performance. Specifically, we characterized the effect of temperature on pollen grain germination in vitro, and on pollen tube growth in vivo in cut flowers and in planta under field temperature conditions. Results obtained showed that temperature variation has a strong effect on pollen germination, and on pollen tube kinetics and on their population size depending on the specific male-female combination. The optimum temperature for pollen germination in vitro was of 25 degrees C, while the most favorable temperature to accelerate in vivo pollen tube growth depended on the particular male-female interaction and ranged between 15 and 25 degrees C. Furthermore, temperature appears to have an effect on self-incompatibility reaction by affecting the place where pollen tubes are arrested. Overall, our results show that pollen performance is not only an inherent characteristic of the pollen genotype, but is largely dependent on the particular male-female combination and on genotype-temperature interactions. (C) 2012 Elsevier B.V. All rights reserved.