A clogging-free microfluidic platform with an incorporated pneumatically driven membrane-based active valve enabling specific membrane capacitance and cytoplasm conductivity characterization of single cells
A clogging-free microfluidic platform with an incorporated pneumatically driven membrane-based active valve enabling specific membrane capacitance and cytoplasm conductivity characterization of single cells
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DOI：
10.1016/j.snb.2013.09.070
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发表时间：
2014-01-01
影响因子：
8.4
通讯作者：
Wu, Min-Hsien
中科院分区：
文献类型：
--
作者：
Huang, Song-Bin;Zhao, Yang;Wu, Min-Hsien
本研究报道了一种用于单电池无堵塞电性能分析的微流控平台。在该平台中集成了气动驱动的膜基主动阀，以疏通收缩微通道的堵塞事件，其中，气动压力用于调节作为收缩微通道一侧的聚二甲基硅氧烷(PDMS)膜的变形。该平台首先在狭窄微通道的入口处对捕获的聚苯乙烯微珠进行了解堵测试，然后成功地展示了肺癌细胞的细胞电学性质的表征。结果表明，测得的细胞质电导率(0.74+/-0.20 S/m)和比膜电容(2.17+/-0.58mU F/cm(2))与前人的结果(分别为0.73+/-0.17 S/m和2.00+/-0.60mU F/cm(2))基本一致。总体而言，这项研究提供了一个用于单细胞分析的微流控平台，该平台具有增强的功能，可以解锁微通道入口处的细胞聚集体。(C)2013爱思唯尔B.V.保留所有权利。
This study reports a microfluidic platform for clogging-free electrical property analysis of single cells. A pneumatically driven membrane-based active valve was integrated in this platform to unblock clogging events of constriction microchannels where pneumatic pressures were used to tune the deformation of a polydimethylsiloxan (PDMS) membrane serving as one wall of the constriction microchannel. The proposed platform was first tested to unblock trapped polystyrene beads at the entrance of constriction microchannels and then the characterization of the cellular electrical properties of lung cancer cells was successfully demonstrated. Results showed that the measured cytoplasm conductivity (0.74 +/- 0.20 S/m) and specific membrane capacitance (2.17 +/- 0.58 mu F/cm(2)) of cells were consistent with the results from the previous publications (0.73 +/- 0.17 S/m, and 2.00 +/- 0.60 mu F/cm(2), respectively). Overall, this study has presented a microfluidic platform for single cell analysis with an enhanced function for unblocking cell aggregates at the entrance of microchannels. (C) 2013 Elsevier B.V. All rights reserved.