Effects of long-term in vitro exposure of ejaculated boar sperm to zearalenone and α-zearalenol in sperm liquid storage medium
Effects of long-term in vitro exposure of ejaculated boar sperm to zearalenone and α-zearalenol in sperm liquid storage medium
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精子液体储存介质中玉米赤霉烯酮和α-玉米赤霉烯醇对射精公猪精子长期体外暴露的影响
DOI：
10.1111/j.1740-0929.2012.01033.x
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发表时间：
2013
影响因子：
2
通讯作者：
J. Fink-Gremmels and T. Otoi
中科院分区：
文献类型：
--
作者：
R. Sambuu;M. Takagi;Z. Namula;M. Nii;M. Taniguchi;S. Uno;E. Kokushi;C. Tshering;R. Rodrigues dos Santos;J. Fink-Gremmels and T. Otoi
通过对猪体外受精（IVF）系统中几个参数的评价，研究了玉米赤霉烯酮（ZEN）和α-玉米赤霉烯醇（α-ZOL）对猪精子体外受精的影响。为此，将猪精子在含有0（对照）、10和1000 µg/L ZEN和α-ZOL的精液储存培养基中于5°C培养1周，用于体外成熟卵母细胞的IVF。 总体而言，不同组精子授精的卵母细胞的总穿透率、单精子受精率和多精子受精率无显著差异。同样，10和1000 µg/L的ZEN和α-ZOL对体外受精后卵母细胞的卵裂和囊胚发育没有不利影响。 尽管与未储存的精子相比，储存3周后精子的活力、活力和质膜完整性显著降低（P<0.05），但即使储存3周后，评价浓度的ZEN和α-ZOL也未对上述参数产生不利影响。 这些结果表明，在代谢减少的条件下，猪精子长期暴露于高达1000 µg/L的ZEN和α-ZOL不会影响其体外功能。
The effects ofin vitroexposure of porcine spermatozoa to zearalenone (ZEN) and α‐zearalenol (α‐ZOL) were studied by evaluating several parameters of anin vitrofertilization (IVF) system. For this purpose, boar spermatozoa cultured with semen storage medium containing 0 (control), 10 and 1000 µg/L of ZEN and α‐ZOL for 1 week at 5°C were used for IVF ofin vitromatured oocytes. Overall, there were no significant differences in the rates of total penetration, monospermic fertilization, and polyspermic fertilization of oocytes inseminated with spermatozoa from the different groups. Similarly, ZEN and α‐ZOL at 10 and 1000 µg/L did not have detrimental effects on the cleavage and development to blastocysts of oocytes afterin vitrofertilization. Although the motility, viability, and plasma membrane integrity of spermatozoa significantly decreased after 3 weeks of storage compared to non‐stored spermatozoa (P< 0.05), ZEN and α‐ZOL at the evaluated concentrations did not exert detrimental effects on the above parameters, even after 3 weeks of storage. These results indicate that prolonged exposure of boar spermatozoa to ZEN and α‐ZOL up to 1000 µg/L under reduced metabolic conditions does not affect theirin vitrofunction.