ADENOSINE-TRIPHOSPHATASE ACTIVITY ASSOCIATED WITH PURIFIED CHOLINERGIC SYNAPTIC VESICLES OF TORPEDO-MARMORATA
ADENOSINE-TRIPHOSPHATASE ACTIVITY ASSOCIATED WITH PURIFIED CHOLINERGIC SYNAPTIC VESICLES OF TORPEDO-MARMORATA
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DOI：
10.1111/j.1432-1033.1977.tb11884.x
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发表时间：
1977-01-01
期刊：
影响因子：
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通讯作者：
WHITTAKER, VP
中科院分区：
文献类型：
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作者：
BREER, H;MORRIS, SJ;WHITTAKER, VP
本文介绍了一种快速纯化电鳐电器官囊泡的方法，该方法采用等渗连续蔗糖-甘氨酸梯度洗脱，然后在CPG-10-3000多孔玻璃珠上层析。突触囊泡的浮力密度为1.057 g/ml。纯化的囊泡不含胆碱酯酶、乳酸脱氢酶和Na+，K+刺激的ATP酶活性。它们含有哇巴因不敏感的Na+，K+抑制，Mg 2+，Ca 2+刺激的ATP酶活性。这是进一步刺激乙酰胆碱，但不是胆碱。
A rapid method for purifying Torpedo electric organ vesicles is described, which employs an isoosmotic continuous sucrose-glycine gradient followed by chromatography on CPG-10-3000 porous glass beads. Synaptic vesicles have a buoyant density of 1.057 g/ml. Purified vesicles are free of cholinesterase, lactate dehydrogenase and Na+,K+-stimulated ATPase activity. They contain a ouabain-insensitive Na+,K+-inhibited, Mg2+, Ca2+-stimulated ATPase activity. This is further stimulated by acetylcholine but not by choline.