Prostate stem cell antigen gene is expressed in islets of pancreas.
Prostate stem cell antigen gene is expressed in islets of pancreas.
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DOI：
10.5115/acb.2012.45.3.149
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发表时间：
2012-09
影响因子：
1.1
通讯作者：
Saeki N
中科院分区：
文献类型：
--
作者：
Ono H;Yanagihara K;Sakamoto H;Yoshida T;Saeki N
前列腺干细胞抗原 (PSCA) 是一种糖基磷脂酰肌醇锚定的细胞表面抗原，在癌症中具有器官依赖性表达模式； e.g., up-regulated in prostate cancer and down-regulated in gastric cancer. Previously it was reported that PSCA is not expressed in the normal pancreas but aberrantly expressed in pancreatic cancer.在本研究中，我们通过免疫组织化学鉴定了胰岛中的 PSCA 表达，该表达与四种胰岛细胞标记物共定位：胰岛素、胰高血糖素、生长抑素和胰多肽。在我们对 PSCA 转录起始位点的研究中，我们在正常胰腺的 mRNA 样本中发现了 PSCA 的非编码剪接变体以及真实的 PSCA 转录本。 Both the transcripts were also identified in several pancreatic cancer cell lines.我们之前报道，在胃癌和胆囊癌细胞系中，PSCA的表达与增强子区域的甲基化状态相关，但在胰腺癌细胞系中则不然，这表明胰腺癌中PSCA表达的调节模式与胃癌和胆囊癌中的不同。
Prostate stem cell antigen (PSCA) is a glycosylphosphatidylinositol-anchored cell surface antigen with an organ-dependent expression pattern in cancers; e.g., up-regulated in prostate cancer and down-regulated in gastric cancer. Previously it was reported that PSCA is not expressed in the normal pancreas but aberrantly expressed in pancreatic cancer. In this present study, we identified PSCA expression in islets of the pancreas by immunohistochemistry, which was co-localized with four islet-cell markers: insulin, glucagon, somatostatin and pancreatic polypeptide. In our investigation of the transcription start site of PSCA, we found a non-coding splicing variant of PSCA as well as authentic PSCA transcripts in mRNA samples from a normal pancreas. Both the transcripts were also identified in several pancreatic cancer cell lines. We previously reported that PSCA expression is correlated to the methylation status of the enhancer region in gastric and gallbladder cancer cell lines but not in pancreatic cancer cell lines, suggesting that PSCA expression is regulated in a diff erent mode in pancreatic cancer from that in gastric and gallbladder cancers.