Lymphoblastoid cell-produced immunoglobulins: preparative purification from culture medium by hydroxylapatite chromatography.
Lymphoblastoid cell-produced immunoglobulins: preparative purification from culture medium by hydroxylapatite chromatography.
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类淋巴母细胞产生的免疫球蛋白：通过羟基磷灰石层析从培养基中制备纯化。
DOI：
10.1016/0003-2697(84)90067-8
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发表时间：
1984
影响因子：
2.9
通讯作者：
Gordon S. Hudson
中科院分区：
文献类型：
--
作者：
Gary J. Smith;Richard D. McFarland;Howard M. Reisner;Gordon S. Hudson
羟基磷灰石（HA）是一种能够分离核酸和蛋白质的色谱基质。HA吸附生物分子作为其表面电荷的程度和分布的函数。已经评价了HA差异保留免疫球蛋白分子的能力，所述免疫球蛋白分子相对于组织培养基中所含的通常球状血清蛋白，特别是白蛋白是平面的。HA色谱法提供了一种从用于补充培养基的大量过量血清蛋白中纯化和浓缩淋巴母细胞样细胞分泌的免疫球蛋白到培养基中的单步方法。人λ轻链蛋白回收到所用培养基体积的5%中，并与总蛋白的95%分离。超过75%的杂交瘤产生的完整免疫球蛋白回收基本上没有血清蛋白污染。HA似乎提供了一种有价值的替代色谱介质，用于纯化由淋巴母细胞样细胞分泌的免疫球蛋白。
Hydroxylapatite (HA) is a chromatography matrix capable of separating nucleic acid as well as protein species. HA adsorbs biomolecules as a function of the extent and distribution of their surface charge. HA has been evaluated for its ability to differentially retain immunoglobulin molecules, which are planar relative to the generally globular serum proteins, particularly albumin, contained in tissue culture medium. HA chromatography provides a single-step method to purify and concentrate immunoglobulin proteins secreted by lymphoblastoid cells into culture medium from the vast excess of serum proteins used to supplement the medium. A human lambda light-chain protein was recovered in 5% of the applied volume of medium, and was separated from 95% of the total protein. More than 75% of a hybridoma-produced complete immunoglobulin was recovered essentially free of serum protein contamination. HA appears to provide a valuable alternative chromatographic medium for the purification of immunoglobulin proteins secreted by lymphoblastoid cells.
测量分离的 X 因子激活系统中的人类 IXa 因子活性。
影响因子：
7.5
作者：
Griffith,MJ;Reisner,HM;Lundblad,RL;Roberts,HR
通讯作者：
Roberts,HR