Inter- and intra-specific gene-density-correlated radial chromosome territory arrangements are conserved in Old World monkeys
Inter- and intra-specific gene-density-correlated radial chromosome territory arrangements are conserved in Old World monkeys
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DOI：
10.1159/000080824
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发表时间：
2005-01-01
影响因子：
1.7
通讯作者：
Mizusawa, H
中科院分区：
文献类型：
--
作者：
Tanabe, H;Küpper, K;Mizusawa, H
最近的研究表明，尽管在进化过程中发生了染色体重排，但人类18和19同源染色体区域（ct）的基因密度相关径向分布在高等灵长类动物中是保守的。然而，这些观察结果仅限于类人猿和新大陆猴物种。为了进一步为基因密度相关CT排列的进化保守性提供证据，我们将先前的研究扩展到旧大陆猴子。为了全面了解高等灵长类人类18和19同源ct的核拓扑结构，它们组成了待分析的剩余物种组。本研究采用三维荧光原位杂交技术（3D-FISH）对三种旧大陆猴的四种淋巴母细胞样细胞系进行了研究：两种日本猕猴（Macaca fuscata）、两种食蟹猕猴（Macaca fascicularis）和一种非洲绿猴（Cercopithecus aethiops）和一种非洲绿猴（Erythrocebus Patas）的种间杂交个体。我们的数据表明，在所有分析的细胞系中，基因贫乏的人18同源ct优先位于靠近核外围的位置，而基因密集的人19同源ct则朝向核中心。人类18和19同源ct的基因密度相关定位在所有主要的高等灵长类谱系中都是进化保守的，尽管在这些物种的进化过程中发生了染色体倒位、融合、分裂或相互易位。这种基因密度相关染色质排列的显著保存为功能相关的高阶染色质结构提供了进一步的支持。版权所有(C) 2005 S. Karger AG，巴塞尔。
Recently it has been shown that the gene-density correlated radial distribution of human 18 and 19 homologous chromosome territories (CTs) is conserved in higher primates in spite of chromosomal rearrangements that occurred during evolution. However, these observations were limited to apes and New World monkey species. In order to provide further evidence for the evolutionary conservation of gene-density-correlated CT arrangements, we extended our previous study to Old World monkeys. They comprise the remaining species group to be analyzed in order to obtain a comprehensive overview of the nuclear topology of human 18 and 19 homologous CTs in higher primates. In the present study we investigated four lymphoblastoid cell lines from three species of Old World monkeys by three-dimensional fluorescence in situ hybridization (3D-FISH): two individuals of Japanese macaque ( Macaca fuscata), crab-eating macaque ( Macaca fascicularis), and an interspecies hybrid individual between African green monkey (Cercopithecus aethiops) and Patas monkey ( Erythrocebus patas). Our data demonstrate that gene-poor human 18 homologous CTs are located preferentially close to the nuclear periphery, whereas gene-dense human 19 homologous CTs are oriented towards the nuclear center in all cell lines analyzed. The gene-density-correlated positioning of human 18 and 19 homologous CTs is evolutionarily conserved throughout all major higher primate lineages, despite chromosomal inversions, fusions, fissions or reciprocal translocations that occurred in the course of evolution in these species. This remarkable preservation of a gene-density-correlated chromatin arrangement gives further support for a functionally relevant higher-order chromatin architecture. Copyright (C) 2005 S. Karger AG, Basel.