Quantitative 3D Micro-CT Imaging of the Human Feto-placental Vasculature in Intrauterine Growth Restriction
Quantitative 3D Micro-CT Imaging of the Human Feto-placental Vasculature in Intrauterine Growth Restriction
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DOI：
10.1016/j.placenta.2008.08.017
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发表时间：
2008-11-01
期刊：
影响因子：
3.8
通讯作者：
Zygmunt, M.
中科院分区：
文献类型：
--
作者：
Langheinrich, A. C.;Vorman, S.;Zygmunt, M.
目的：胎盘血管发育与胎儿生长发育相匹配。妊娠胎盘中胎儿胎盘脉管系统的量化由于宫内生长受限（IUGR）而变得复杂，结果令人困惑。因此，通过 3D 微型计算机断层扫描 (micro-CT) 评估 IUGR 胎盘中的胎儿胎盘血管体积。 方法和结果：使用 Microfil OF BaSO4 对 IUGR (n = 24) 和健康对照胎盘 (n = 40) 的胎盘探针进行原位灌注，并通过 micro-CT 扫描随机选择的样本。使用 3D 图像，我们定量了胎儿胎盘血管体积分数 (VVF)。在三个不同级别进行亚分析，从绒毛膜板动脉（A 级）到中间动脉（B 级）和毛细血管系统（C 级）。结果得到组织学的补充。通过方差分析 [ANOVA] 测试了血管体积测量值差异的显着性。结果：Microfil 灌注胎盘显示健康对照中的总血管体积分数为 20.5 +/- 0.9%。相比之下，IUGR 胎盘的 VVF 下降至 7.9 +/- 0.9% (p < 0.001)。使用显微 CT 和组织学发现 Microfil 和 BaSO4 灌注胎盘的血管体积分数存在显着差异。 Micro-CT显示并发IUGR的胎盘中间动脉出现局部同心管腔侵犯。结论：Micro-CT成像可用于健康对照和并发IUGR妊娠的胎儿胎盘血管树的定量分析。 (C) 2008 Elsevier Ltd. 保留所有权利。
Objective: Placental vascular development matches fetal growth and development. Quantification of the feto-placental vasculature in placentas from pregnancies is complicated by intrauterine growth restriction (IUGR) revealed confounding results. Therefore, the feto-placental vascular Volume in IUGR placentas was assessed by 3D micro-computed tomography (micro-CT).Methods and results: Placental probes from IUGR (n = 24) and healthy control placentas (n = 40) were perfused in situ with Microfil OF BaSO4 and randomly chosen samples were scanned by micro-CT. Using 3D images, we quantitated the feto-placental vascular Volume fraction (VVF). A subanalysis was per-formed at three different levels, reaching from the chorionic plate artery (level A), to intermediate arteries (level B) and capillary system (level C). Results were complemented by histology. The significance of differences in vascular volume measurements was tested with analysis of variance [ANOVA].Results: Microfil perfused placentas showed a total vascular volume fraction of 20.5 +/- 0.9% in healthy controls. In contrast, the VVF decreased to 7.9 +/- 0.9% (p < 0.001) in IUGR placentas. Significant differences were found between Microfil and BaSO4 perfused placentas in the vascular Volume fraction using micro-CT and histology. Micro-CT demonstrated localized concentric luminal encroachments in the intermediate arteries in placentas complicated by IUGR.Conclusion: Micro-CT imaging is feasible for quantitative analysis of the feto-placental vascular tree in healthy controls and pregnancies complicated by IUGR. (C) 2008 Elsevier Ltd. All rights reserved.