CjPLE, a PLENA-like gene, is a potential regulator of fruit development via activating the FRUITFUL homolog in Camellia
CjPLE, a PLENA-like gene, is a potential regulator of fruit development via activating the FRUITFUL homolog in Camellia
复制标题
CjPLE 是一种 PLENA 样基因，通过激活山茶花中的 FRUITFUL 同源物，成为果实发育的潜在调节因子
DOI：
10.1093/jxb/erz142
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发表时间：
2019-06-01
影响因子：
6.9
通讯作者：
Yin, Hengfu
中科院分区：
文献类型：
--
作者：
Lyu, Tao;Fan, Zhengqi;Yin, Hengfu
果实形成涉及多个过程的合作，包括代谢变化、细胞分化和细胞扩增。FRUITFUL（FUL）和SHATTERPROOF 1/2（SHP）MADS盒基因是几种真双子叶植物中指导果实图案形成的主调节因子。然而，FUL-SHP网络在不同水果类型中的调控机制仍不清楚。在这里，我们的特点是功能的直系同源物（CjPLE）的SHP从山茶。结果表明，CjPLE基因在花发育早期主要表达于雄蕊和心皮组织，在果实发育过程中，CjPLE基因在果皮组织中大量表达。CjPLE在拟南芥中的异位表达引起心皮的发育增强，而没有观察到花的身份缺陷。为了研究CjPLE的下游靶标，通过杜鹃红山茶中的愈伤组织转化系统分析过表达转化体。我们检测了潜在下游靶基因的表达水平，发现两个先前鉴定的APETALA 1样基因（CjAPL 1/2）显著上调。我们表明，CjPLE直接结合到CjAPL 1（FUL直系同源物）的启动子区的CArG基序。综上所述，我们的研究结果揭示了一个可能的正调控FUL由SHP在控制果实发育的山茶。
Fruit patterning involves the cooperation of multiple processes, including metabolic change, cell differentiation, and cell expansion. The FRUITFUL (FUL) and SHATTERPROOF1/2 (SHPs) MADS-box genes are master regulators directing fruit patterning in several eudicots. However, the regulatory mechanisms of the FUL-SHP network in different fruit types remain unclear. Here, we characterized the functions of an ortholog (CjPLE) of SHPs from Camellia japonica. We showed that CjPLE was predominantly expressed in stamen and carpel tissues during the early stage of floral development and that transcripts were abundant in the pericarp tissues during fruit development. The ectopic expression of CjPLE in Arabidopsis caused enhanced development of the carpels, whereas no defects in floral identity were observed. To investigate the downstream targets of CjPLE, overexpression transformants were analysed through a callus transformation system in Camellia azalea. We examined the expression levels of potential downstream target genes and found that two previously identified APETALA1-like genes (CjAPL1/2) were significantly up-regulated. We showed that CjPLE directly bound to the CArG motifs in the promoter region of CjAPL1 (the FUL ortholog). Taken together, our results reveal a possible positive regulation of FUL by SHP in the control of fruit development in Camellia.