Perivascular niche of postnatal mesenchymal stem cells in human bone marrow and dental pulp
Perivascular niche of postnatal mesenchymal stem cells in human bone marrow and dental pulp
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DOI：
10.1359/jbmr.2003.18.4.696
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发表时间：
2003-04-01
影响因子：
6.2
通讯作者：
Gronthos, S
中科院分区：
文献类型：
--
作者：
Shi, S;Gronthos, S
间充质干细胞群先前已在成人骨髓和牙髓中鉴定出，其能够分别再生骨髓和牙髓微环境。在这里，我们表明，这些干细胞群体驻留在其组织的起源微血管。人骨髓基质干细胞（BMSSC）和牙髓干细胞（DPSC）通过使用抗体STRO-1的免疫选择分离，STRO-1识别骨髓和牙髓组织中血管周围细胞上的抗原。通过免疫组织化学、荧光激活细胞分选（FACS）和/或免疫磁珠选择，测试新鲜分离的STRO-1阳性BMSSC和DPSC的已知由内皮细胞（血管性血友病因子，CD 146）、平滑肌细胞和周细胞（α-平滑肌肌动蛋白，CD 146）表达的血管抗原和周细胞相关抗原（3G 5）的表达。BMSSC和DPSC均缺乏血管性血友病因子的表达，但发现α-平滑肌肌动蛋白和CD 146阳性。此外，大多数DPSC表达周细胞标记物3G 5，而仅发现少数BMSSC群体对3G 5呈阳性。BMSSC和DPSC都显示出与不同血管周围细胞群一致的表型，无论其不同的个体发育和发育潜力如何，这一发现可能对理解调节矿化基质和其他相关结缔组织形成的因素有进一步的影响。
Mesenchymal stem cell populations have previously been identified in adult bone marrow and dental pulp that are capable of regenerating the bone marrow and dental pulp microenvironments, respectively. Here we show that these stem cell populations reside in the microvasculature of their tissue of origin. Human bone marrow stromal stem cells (BMSSCs) and dental pulp stem cells (DPSCs) were isolated by immunoselection using the antibody, STRO-1, which recognizes an antigen on perivascular cells in bone marrow and dental pulp tissue. Freshly isolated STRO-1 positive BMSSCs and DPSCs were tested for expression of vascular antigens known to be expressed by endothelial cells (von Willebrand factor, CD146), smooth muscle cells, and pericytes (alpha-smooth muscle actin, CD146), and a pericyte-associated antigen (3G5), by immunohistochemistry, fluorescence-activated cell sorting (FACS), and/or immunomagnetic bead selection. Both BMSSCs and DPSCs lacked expression of von Willebrand factor but were found to be positive for alpha-smooth muscle actin and CD146. Furthermore, the majority of DPSCs expressed the pericyte marker, 3G5, while only a minor population of BMSSCs were found to be positive for 3G5. The finding that BMSSCs and DPSCs both display phenotypes consistent with different perivascular cell populations, regardless of their diverse ontogeny and developmental potentials, may have further implications in understanding the factors that regulate the formation of mineralized matrices and other associated connective tissues.